. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 158 DOROTHY E. BLISS AND JOHN H. WELSH A modification of the conventional methylene blue technique for nerve differen- tiation has proved useful. The eyestalk ganglia and brain, stripped of their con- nective tissue sheath, are placed in the well of a depression slide, containing about 20 drops of perfusion medium and two drops of methylene blue. After sev- eral minutes the preparation is examined against a black background in the same amount of perfusion medium and two drops of glycerol. Alternate staining and cleari
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 158 DOROTHY E. BLISS AND JOHN H. WELSH A modification of the conventional methylene blue technique for nerve differen- tiation has proved useful. The eyestalk ganglia and brain, stripped of their con- nective tissue sheath, are placed in the well of a depression slide, containing about 20 drops of perfusion medium and two drops of methylene blue. After sev- eral minutes the preparation is examined against a black background in the same amount of perfusion medium and two drops of glycerol. Alternate staining and clearing yields a preparation in which nerve cells appear deep blue and nerve tracts white with blue edges. Since it is known that both neurosecretory and other. -PLO —CC la. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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