. Pathological technique; a practical manual for workers in pathological histology and bacteriology. 6. Wash in water. 7. Stain in saturated aqueous solutionof Bismarck brown for thirty seconds. 8. Wash in water and method is nothing but Grams method and after staining with Bis-marck brown. With it the gonococciare stained brown, and other pyogeniccocci blue-black. W. F. Whitney has suggested theuse of a 1 per cent, aqueous solutionof pyronin in place of the solution ofBismarck brown in step 7. The gono-cocci are stained red by the pyronin. Pappenheims Method. — 1. Stainsmear prepar
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. 6. Wash in water. 7. Stain in saturated aqueous solutionof Bismarck brown for thirty seconds. 8. Wash in water and method is nothing but Grams method and after staining with Bis-marck brown. With it the gonococciare stained brown, and other pyogeniccocci blue-black. W. F. Whitney has suggested theuse of a 1 per cent, aqueous solutionof pyronin in place of the solution ofBismarck brown in step 7. The gono-cocci are stained red by the pyronin. Pappenheims Method. — 1. Stainsmear preparation for three to fiveminutes in the following mixture:Methyl green,Pyronin,5 per cent, carbolic acid water, 2. Wash off in water; dry; mount in xylol balsam. Nuclei green; cocci bright red. For demonstrating the gonococcus in sections of tissuesthe general stains used for Gram-negative bacteria givegood results. After Zenkers fixation Mallorys eosin andmethylene-blue method is recommended. Micrococcus Catarrhalis.—This micrococcus may befound in the sputum in inflammatory conditions of the. Fig. 51.—Micrococcuscatarrhalis colonies onagar (F. T. Lord; photoby L. S. Brown). 2 grams;2 IOO 278 PA THOL O GICAL TE CHNIQ UE. respiratory tract and cannot be distinguished in its mor-phology and staining reactions from the gonococcus or fromthe Diplococcus intracellularis meningitidis (Fig. 53). Theappearances of its colonies on ordinary culture-media are,however, characteristic. They are large, white, of irregularoutline, and have elevated central portions. They are friable,not viscid, and grow readily at room-temperature (Fig. 51). Micrococcus TetragentlS.—The colonies are small,white, and elevated. Growth is slow. Morphology.—Micrococci arranged in fours, or tetrads,held together by a gelatinous substance (Fig. 52). Stained by Grams method. Not motile. Gelatin Stab.—Feeble growth in the form of minute spheri-cal masses along the line of stab with a small white, slig
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