. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. 282 ELECTRON TRANSPORT Nijenhuis (23). Comparison of the action spectrum with the absorption spectrum indicates that the efficiency of light absorbed by carotenoids is approximately 40 per cent of that absorbed by bacteriochlorophyll, in good agreement with Goedheer (24),. 0,3 400 450 500 550 WAVELENGTH (myj) 600 Fig. 5. Action spectrum of light inhibition on respiration of "young" light- grown cells of R. ntbno)/. Before and after this experiment, it was determined with the use of the oxygen-electro
. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. 282 ELECTRON TRANSPORT Nijenhuis (23). Comparison of the action spectrum with the absorption spectrum indicates that the efficiency of light absorbed by carotenoids is approximately 40 per cent of that absorbed by bacteriochlorophyll, in good agreement with Goedheer (24),. 0,3 400 450 500 550 WAVELENGTH (myj) 600 Fig. 5. Action spectrum of light inhibition on respiration of "young" light- grown cells of R. ntbno)/. Before and after this experiment, it was determined with the use of the oxygen-electrode method that the ratios of dark-to-light respiration were from to at 27°C. The action spectrum was measured by an apparatus which was devised by Castor and Chance (21). Other experi- mental conditions were the same as for Fig. 1, except that pH of the cell sus- pension was The absorption spectrum was measured with a Cary, model 14R, spectrophotometer, using a chromatophore suspension prepared by the conventional 90-second sonication method (chromatophores (S)) (20). The cells, particularly if aged under aerobic conditions or disrupted, started to show the oxygen uptake which was stimulated under illumi- nation, Taylor (unpublished results) has found that the action spectrum for light-stimulated respiration with the aged, light-grown cells shows peaks between 420 m/i and 430 m/i, much as in Fig. 6. He also obtained an action spectrum with the aged, blue-green mutant (devoid of caro- tenoids) of Rhodopseudonioiias spheroides, which showed a prominent shoulder at 410 m^ on a peak somewhere in the near ultraviolet and a small symmetrical peak at 520 vaid. It may be worth noting that these. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Symposium on Bacterial Photosynthesis (1963 : Yellow Springs
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