. Biophysical science. Biophysics. 26 : 3/ Absorption Spectrophotometry 493 spectral dispersion. There is little or no inherent advantage (or dis- advantage) in a grating rather than a prism for a spectrophotometer used to measure the absorption of biologically interesting Heated Filament Eye or Other Detector Figure 8. Simplified diagram of a prism monochromator. Both can be calibrated far more precisely than has any meaning in bio- logical studies, and both can be designed to pass comparable amounts of light at the same wavelength bandwidth. A grating spectrophotometer is schemat
. Biophysical science. Biophysics. 26 : 3/ Absorption Spectrophotometry 493 spectral dispersion. There is little or no inherent advantage (or dis- advantage) in a grating rather than a prism for a spectrophotometer used to measure the absorption of biologically interesting Heated Filament Eye or Other Detector Figure 8. Simplified diagram of a prism monochromator. Both can be calibrated far more precisely than has any meaning in bio- logical studies, and both can be designed to pass comparable amounts of light at the same wavelength bandwidth. A grating spectrophotometer is schematically illustrated in Figure 9 for a transmission type grating. Light generated by the heated filament passes through slit Sx located in the focal plane of lens Lx. The parallel light so produced falls on the grating G. Each line of the grating acts as a source of light giving rise to diffracted rays which are focused by the lens L2 onto the plane of the slits S2. The path lengths Red. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ackerman, Eugene, 1920-. Englewood Cliffs, N. J. , Prentice-Hall
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