. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 1. Morphology ami microanatomy oj dog/ish /eases exposed to V\'-A radiation with or without modifiers, ti) Opacities in whole lenses after 15 h of incubation: Loner row licit lo right) dark control: with mM HT; with I mm HT: \\-ilh 10 mM HT: upper row (left to rig/ill I I - c\poscJ; with mM HT: with 1 mM HT ami with 10 mM HT (h-1) Rhodamine-phalloidin stained dogfish lens epithelial cells in whole mount (magnification 5?50x) after 15 h incubation, b) Dark control: c) UV- exposed;d) L'l '-exposed plus a-tocophe
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 1. Morphology ami microanatomy oj dog/ish /eases exposed to V\'-A radiation with or without modifiers, ti) Opacities in whole lenses after 15 h of incubation: Loner row licit lo right) dark control: with mM HT; with I mm HT: \\-ilh 10 mM HT: upper row (left to rig/ill I I - c\poscJ; with mM HT: with 1 mM HT ami with 10 mM HT (h-1) Rhodamine-phalloidin stained dogfish lens epithelial cells in whole mount (magnification 5?50x) after 15 h incubation, b) Dark control: c) UV- exposed;d) L'l '-exposed plus a-tocopherol added at -5 tiM: el dark control plus 10 mM OH-TEMPO: I) Ul'-c.\poscd plus 10 mM OH-TEMPO added depleted in the UV-exposed lenses by about 30-40% compared with dark controls. Thus, UV-A exposure lowered the ATP level concomitant with opacity formation. UV-A-exposed lens epithelial cells contained few if any actin filaments, whereas in the dark controls filaments were prominent (see Fig. Ib, c). The degree of loss of these actin filaments due to UV-exposure was lessened when vitamin E was present in the medium (see Fig. Id). However, OH-TEMPO caused a greater opacity and a greater degree of alteration of the actin filaments (Fig. le, f). When OH-TEMPO was added at 10 mM, a totally aberrant pattern of actin distribution was observed. The fluorescence due to phalloidin-rhodamine binding to actin was concentrated in intense spots near the lens cell membranes and interconnecting rays of filaments, forming star-like figures. Thus, opacification, ATP depletion, and F-actin breakdown all result from UV-A irradiation. Purified rabbit muscle F-actin was also degraded by 5-20% into monomeric fragments due to UV-irradiation. HPLC data indicated that cv-tocopherol (at 25 fiAI) neither protected nor enhanced UV-induced F-actin degradation. We conclude that a photooxidative process degrades filamen- tous actin, and that a-tocopherol partially protects it in cells, whereas OH-TEMPO (the sp
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
