. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 512 M. J. FAIN AND L. M. RIDDIFORD LU or o o CO < LJ 0-*. -5 -4 -3 -2 LOG HORMONE DOSE 0 FIGURE 2. Dose-response relations for C16- and C18JH in the black mutant pigmenta- tion assay. Squares represent C18JH; circles, C16JH; solid lines, best-fit lines determined by linear regression of individual scores onto dose, at all doses for which mean score > (slopes and , respectively) ; dotted lines, 95% confidence limits. Correlation co- efficients are C18JH, R = ; C16JH, R = X-intercepts are C18JH, 2.
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 512 M. J. FAIN AND L. M. RIDDIFORD LU or o o CO < LJ 0-*. -5 -4 -3 -2 LOG HORMONE DOSE 0 FIGURE 2. Dose-response relations for C16- and C18JH in the black mutant pigmenta- tion assay. Squares represent C18JH; circles, C16JH; solid lines, best-fit lines determined by linear regression of individual scores onto dose, at all doses for which mean score > (slopes and , respectively) ; dotted lines, 95% confidence limits. Correlation co- efficients are C18JH, R = ; C16JH, R = X-intercepts are C18JH, X lO'5 /tg; C16JH, X 10"3 Each point represents the mean score of 25-37 larvae; the bars indicate ± one standard deviation. Triangles show mean scores for 3 dilutions of C18JH "extracted" and assayed according to the procedures in Methods (9 ± 1 assay larvae per point). was similar to that observed in the original pigmentation assay utilizing neck- ligated wild-type larvae (Truman et al., 1973) in that the larval cuticle appeared hormone application. Dose-response relations ]or juvenile hormones Figure 2 shows the mean scores obtained for different doses of C16- and C18JH. The lines drawn through each set of points are the least squares lines calculated by regression of individual scores onto the logic hormone dose. The correlation :oefficients (R == and R = for CIS- and C16JH, respectively) indicate that these lines well represent the experimental data. As expected from results m other Manduca JH assays (Nijhout and Riddiford, 1974; Riddiford and Ajami, 1973), C16JH is considerably less active than C1SJH. In spite of a nearly 200-fold difference in the activities of these two hormones, the slopes of their respective. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Mari
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
