. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 500 A 43° 45'N - 43° 10'N- -69° 30'W -68° 55'W _l i -68° 20'W . 14 4 ' •'''•' /••?",-VV Cell Number cells/I 0 0 or non-detectable. Figure 1. Spcci/iiit\ l' Uirtic rihosomal snbunil ILSU) gene primer?, ami a imii> «t AlcxanJrium fundyense distribution. (At The arrow indicates a 174-hp fragment of the LSU gem: as shown In tin- Illtl-hp laddci Amplification occurred nnl\ in strains of A. fundyense that are present in the Gulf of Maine (GTCA28. CB30I, GTPPOIl. Amp/iln ation c/icl nut incur in Alexandrium \tiams I rum oth
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 500 A 43° 45'N - 43° 10'N- -69° 30'W -68° 55'W _l i -68° 20'W . 14 4 ' •'''•' /••?",-VV Cell Number cells/I 0 0 or non-detectable. Figure 1. Spcci/iiit\ l' Uirtic rihosomal snbunil ILSU) gene primer?, ami a imii> «t AlcxanJrium fundyense distribution. (At The arrow indicates a 174-hp fragment of the LSU gem: as shown In tin- Illtl-hp laddci Amplification occurred nnl\ in strains of A. fundyense that are present in the Gulf of Maine (GTCA28. CB30I, GTPPOIl. Amp/iln ation c/icl nut incur in Alexandrium \tiams I rum other pun-. «f the world (SP3B5. AL8T. TN9A): nor did amplification occur in oilier Alexandrium species \nch n\ A. ostenfeldii ami A. andersoni that could occur with A. fundyense in the Gulf of Maine (A. ostenfeldii, TC02). Other Jinoflagellate species also did not amplify (CCMPIV37, SA2. Karenia breve, Prorocentrum minimum, GPES22). (B> A map of A. fundyense distribution in surface water of the Gull "/ Manic during a cruise from 2V May to 6 June 21103. and a 60 °C annealing temperature. Under these conditions ampli- fication occurred only with A. finnlvfiisc isolated from the Gulf of Maine (Fig. 1A). Field samples were collected from surface water along transects in the Gulf of Maine from 29 May through 6 June 21)03. At each station, 4 1 of surface water was collected, prescreened through a sieve, and collected on a 15-jum filter. The samples were each extracted with Qiagen DNEasy kit. Using qPCR, the number of cells in a field sample was deter- mined. In this study, qPCR was performed using Strutagene Bril- liant SYBR Green QPCR Master Mix. and a fluorescence thresh- old was set by the analytical software for the BioRad iCycler. The PCR cycle during which this threshold was crossed for each sample designated the Cr. Sample C, can be compared to the Cr of standards with a known cell count to specify the number of cells present in the sample (4). To
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
