Studies in blood-pressure : physiological and clinical . d withcotton, having been accurately rilled and cleared fromadherent blood by the finger, the rubber nozzle of themixer filled with Hayems solution is then appliedover its bevelled end, and the blood is thoroughlywashed into the flattened tube (Fig. 9). When theblood is not suspected to be particularly poor in haemo-globin and corpuscles, the amount of fixing fluid addedin the first instance may raise the column to the 60 or70 per cent, mark ; otherwise the first charge shouldbe less. The contents of the tube are then uniformly 1 The for
Studies in blood-pressure : physiological and clinical . d withcotton, having been accurately rilled and cleared fromadherent blood by the finger, the rubber nozzle of themixer filled with Hayems solution is then appliedover its bevelled end, and the blood is thoroughlywashed into the flattened tube (Fig. 9). When theblood is not suspected to be particularly poor in haemo-globin and corpuscles, the amount of fixing fluid addedin the first instance may raise the column to the 60 or70 per cent, mark ; otherwise the first charge shouldbe less. The contents of the tube are then uniformly 1 The formula is as follows: Hydrargyri perchloridum, gramme05; sodii chlorid., gramme TO ; sodse sulphas, grammes 50 ;aqua distillata, 200 230 APPENDIX mixed by inverting the tube a few times with thethumb over its mouth, care being taken on removingthe thumb to draw it over the lip of the tube so as torestore as much as possible the fluid adhering to procedure should of course be repeated afterevery addition of the diluting fluid. The observation. Fig. -washing the blood into the hemacytometer tubewith Hayems Solution. is made in a dark room or in a darkened room freefrom cross-lights. The observer stands ten feet fromthe flame. In making the observation it is importantto shut out as much as possible the diffused light of thecandle. The most satisfactory way to do this is toplace the lower end of the tube, with its long diameter THE HEMACYTOMETER 231 in a line with the candle, in the concavity betweenthumb and forefinger, so as to make as it were a framefor it, and then to bring this part of the tube quiteclose to the eye (Fig. 8,d). The observer must look outsharply for the first appearance of the faint transverseline of light which dilution brings into view, and tneearliest indications are obtained by turning the tubeon its axis, when the line will first become visible atthe sides of the tube. II. The Lymphometer The hemacytometer, adapted to the measure-ment of tis
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