. Auxins and plant growth. Auxin; Plant growth promoting substances. 32 Fundamentals of Auxin Action and cover with dry vermiculite or sand to a depth of about two inches. Place in the darkroom (25° C). Planting should be eight days before the test. The seedlings must receive some red light. Three hours of red light per day is very satisfactory. Cutting Select seedlings in which the stem above the first leaf node (the 3rd node) is i/^ to i/o inch long. Uniformity of plants used is important. Cut off the seedlings about 2 inches from the top, decapitate 14 inch below the leaf node, and slit the
. Auxins and plant growth. Auxin; Plant growth promoting substances. 32 Fundamentals of Auxin Action and cover with dry vermiculite or sand to a depth of about two inches. Place in the darkroom (25° C). Planting should be eight days before the test. The seedlings must receive some red light. Three hours of red light per day is very satisfactory. Cutting Select seedlings in which the stem above the first leaf node (the 3rd node) is i/^ to i/o inch long. Uniformity of plants used is important. Cut off the seedlings about 2 inches from the top, decapitate 14 inch below the leaf node, and slit the stem from the tip to standard distance (3 cm.). For uniform results it is essential to have stems slit exactly down the middle. Wash Place the slit sections in a dish of distilled water for one hour. Place in solutions Make up the solutions to be tested at a volume of 25 ml. in 100 X 20 mm. Petri dishes. Place 5 or 6 slit peas in each dish, choosing carefully for symmetry and uniformity of negative (outward) curvature. Do not use stems with less than 60° negative curvature on each Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Leopold, A. Carl (Aldo Carl), 1919-. Berkeley, University of California Press
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