. dan bai zhi jie gou fen xi zhi bei jian ding yu wei liang ce xu. botany. æ³çèåï¼å ¶ç®çæ¯ä¸ºäºè·å¾è¶³å¤å¤çé«äº§çå¤è½ä»èå¯è½ç¨äºä¸çº§ç»æçåæå·¥ä½ã é解æ¹å¼çéæ©ä¸»è¦åå³äºæéè¦ç段çæ°éå大å°ï¼Matsudaira 1993)ï¼å¨ä½å«é æ®åºï¼å¦Metï¼ Asp-ProæTip)çä½ç½®ä¸è¿è¡åå-¦é解æ¶ï¼å èµ°SDS-PAGE (Tricine-è¶ï¼ Schagger and von Jagow 1987),ç¶åå°åä¸è½ç带çµè½¬å°æ°æ§èä¸ï¼èäºä¹ç¨ï¼PVDF), å¯è½æ¯é²æ-¢å¾®éèç½æ ·åæ失çæä½³éæ©æ¹æ³ï¼ç¸åå°ï¼å°éæ ·åå¨é«åºç°é¢çæ®åº (ä¾å¦LysæAi?)çä½ç½®ä¸è¿è¡èç½é ¶è§£åç产ç©åæ好æ¯éç¨ç»çåç¸HPLCæ±è¿è¡ å离ã åå-¦åé ¶è§£è¯å æ常ç¨çåå-¦æ¹æ³æ¯ç¨CNBrç70%ç²é ¸æä¸æ°ä¹é ¸æº¶æ¶²å¨ç²ç¡«æ°£é ¸ç羧端å¤å
. dan bai zhi jie gou fen xi zhi bei jian ding yu wei liang ce xu. botany. æ³çèåï¼å ¶ç®çæ¯ä¸ºäºè·å¾è¶³å¤å¤çé«äº§çå¤è½ä»èå¯è½ç¨äºä¸çº§ç»æçåæå·¥ä½ã é解æ¹å¼çéæ©ä¸»è¦åå³äºæéè¦ç段çæ°éå大å°ï¼Matsudaira 1993)ï¼å¨ä½å«é æ®åºï¼å¦Metï¼ Asp-ProæTip)çä½ç½®ä¸è¿è¡åå-¦é解æ¶ï¼å èµ°SDS-PAGE (Tricine-è¶ï¼ Schagger and von Jagow 1987),ç¶åå°åä¸è½ç带çµè½¬å°æ°æ§èä¸ï¼èäºä¹ç¨ï¼PVDF), å¯è½æ¯é²æ-¢å¾®éèç½æ ·åæ失çæä½³éæ©æ¹æ³ï¼ç¸åå°ï¼å°éæ ·åå¨é«åºç°é¢çæ®åº (ä¾å¦LysæAi?)çä½ç½®ä¸è¿è¡èç½é ¶è§£åç产ç©åæ好æ¯éç¨ç»çåç¸HPLCæ±è¿è¡ å离ã åå-¦åé ¶è§£è¯å æ常ç¨çåå-¦æ¹æ³æ¯ç¨CNBrç70%ç²é ¸æä¸æ°ä¹é ¸æº¶æ¶²å¨ç²ç¡«æ°£é ¸ç羧端å¤åæ- è½é¾ï¼è¿ä¸ªæ¹æ³ç主è¦ä¼ç¹æ¯å¯å¾ç®åå°éè¿å¾®éç空离å¿æºæµç¸®å»é¤æ¥åç©åå¯äº§ ç©ï¼å¹¶ä¸ï¼å¦æå°å-å¨äºåè¶ä¸-æåºå®å¨èä¸çèç½è¿è¡åä½é解æ¶ï¼åéç¨70%ç² é ¸è¿ç§å¾å¥½çå¤è½æº¶åï¼å¾å®¹æå°ç段ååºæä»åè¶æPVDFèä¸æ½æè³ä¸æ¸ ä¸-ãå¦æ åç°å¯¹æ转移èç½çè½çåæ¶ç太ä½ï¼ä¾å¦ï¼é常é¿çæçæ°´çè½æ®µå¯è½ä¿æå¨è½½ä½ ä¸ï¼åæ好æ¯å°å¨PVDFèä¸çç段ç¨èç½é ¶åé ¶è§£ä¸æ¬¡ã æ»ç»æç®ä¸-å·²æçé ¶è§£åºç¨ï¼è½ç¶è°èç½é ¶æ¯å¨èç½è´¨åä½é解ä¸-éç¨æå¤çèã ä½è°åä¹³èç½é ¶ãèç½å åè¤ãLys-CåAsp-Nä¹åæ ·ææï¼Fernandez et al. 1992)ãå¤ ç§èç½é ¶çææ使ç¨å¯ä»¥æ¹åè·å¾å¤§å°åéè½æ®µçæºä¼ï¼15个氨åºé ¸å·
Size: 2090px × 1196px
Photo credit: © The Book Worm / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcollectionbiodiversity, bookcontributorli, booksubjectbotany
