. Biological studies by the pupils of William Thompson Sedgwick. Published in commemoration of the twenty-fifth anniversary of his doctorate. Biology. Experiments with Bacterial Enzymes 133 in a 5 per cent acid medium, while in a neutral solution 50 M. L. D. were completely destroyed. That acid and alkali are not the only substances whose presence must affect the "heat resistance" of EXPERIMENT. Broth Culture B. prodigiosus. UNHEATED. Amt. of Culture c. c. Acid 1% Acid Grig. React. Neutral Alka- line + + + + + + + + + ± + + 4- + 0 + + + + 0 HEATED 7a


. Biological studies by the pupils of William Thompson Sedgwick. Published in commemoration of the twenty-fifth anniversary of his doctorate. Biology. Experiments with Bacterial Enzymes 133 in a 5 per cent acid medium, while in a neutral solution 50 M. L. D. were completely destroyed. That acid and alkali are not the only substances whose presence must affect the "heat resistance" of EXPERIMENT. Broth Culture B. prodigiosus. UNHEATED. Amt. of Culture c. c. Acid 1% Acid Grig. React. Neutral Alka- line + + + + + + + + + ± + + 4- + 0 + + + + 0 HEATED 7a " FOR I HOUR * + + + ± 0 + ± 0 0 0 0 0 0 0 0 0 0 0. 06 0 ^ 0 0 *In each case 2 of culture or filtrate was placed in a sealed tube and immersed in a water-bath. EXPERIMENT. Broth Culture B. pyocyaneus. UNHEATED. Amt. of Culture 3% Acid Acid Original Neutral 0. 20 -t- -1- -f- 0 + + -1- + 0 + + + + 0 HEATED TO 70° FOR 30 MINUTES, an enzyme is obvious. In some cases a neutral broth culture was found to resist heating better than a neutral gelatin culture, that is, 2 M. L. D. of the gelatin culture were destroyed at a lower tempera- ture. Among the great variety of substances produced by bacteria, there must be many whose presence affects the stability of the enzyme when heat is applied. Any accurate determination of the heat resist- ance of bacterial enzymes is hardly possible when the enzymes are. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Sedgwick, W. T. (William Thompson), 1855-1921; Ayers, S. Henry; Calkins, Gary N. (Gary Nathan), b. 1869; Dyar, Harrison G. (Harrison Gray), 1866-1929; Fuller, George Warren, 1868-; Gage, Stephen De Meritte, 1874-; Ham, Clara E; Hough, Theodore, 1865-; Jackson, Daniel Dana, 1870-; Jordan


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Keywords: ., bookcentury1900, bookdecade1900, booksubjectbiology, bookyear1906