. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PROSOBRANCH OSPHRAD1UM 175. Figure 1. Scanning electron micrographs of the osphradium in the prosobranchs studied. A. Littorina littorca. A transverse section has been made through the osphradium. Asterisk labels the ganglion, and the arrow indicates the lateral ciliated zone of the ridge. B. Ampullarius sp. C. Biimnum midaiiim. Scale bars: A = 100 Mm; B. C = 200 /im. bipectinate osphradium—in Biiccinum undatum. Our comparative study also included the morphologically pe- culiar organ of an ampullariid snail (Haszprunar, 1985
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PROSOBRANCH OSPHRAD1UM 175. Figure 1. Scanning electron micrographs of the osphradium in the prosobranchs studied. A. Littorina littorca. A transverse section has been made through the osphradium. Asterisk labels the ganglion, and the arrow indicates the lateral ciliated zone of the ridge. B. Ampullarius sp. C. Biimnum midaiiim. Scale bars: A = 100 Mm; B. C = 200 /im. bipectinate osphradium—in Biiccinum undatum. Our comparative study also included the morphologically pe- culiar organ of an ampullariid snail (Haszprunar, 1985) and the mantle epithelia surrounding the prosobranch os- phradium as well as that of a pelecypod, the freshwater mussel Anodonta cygnea, which has a poorly developed osphradium. Materials and Methods Specimens of the periwinkle Littorina littorea (Proso- branchia, Mesogastropoda. Littorinoidea), cm long, and the whelk Biiccinum undatum (Prosobranchia, Neo- gastropoda, Buccinoidea). 7-9 cm long, were collected in the wild at the Kristineberg Marine Biological Station on the west coast of Sweden, and maintained in aquaria for about two weeks at about 10°C. Specimens of the fresh- water snail Ampullarius sp. (Prosobranchia, Mesogastro- poda, Viviparoidea) and the pelecypod Anodonta cygnea were from an aquarial culture. To dissect osphradia and surrounding tissues, animals were anesthetized in an iso- tonic solution of MgCU for 20 min and then extracted from their shells. Scanning electron microscopy The osphradia were fixed in glutaraldehyde (TAAB) in M Na-cacodylate buffer, pH (23 mg/ ml NaCl were added for the marine species), for 2 h, and washed in the same buffer for 2 h. To remove mucus from the surface, the specimens were treated for 6 h with leech hyaluronidase (Sigma), 50-100 units/ml, in the same buffer. All steps were at room temperature. Finally, the specimens were dehydrated in an ethanol series, dryed in Balsers Union Critical Point Dryer, fix
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
