The Journal of biological chemistry . ents were carried out,however, and the results are shown in Figs. 4 and 5. In one case5 per cent of the organisms was added to the ration and in theother 10 per cent was used to replace a like amount of starch. 2 The culture of Bacillus timothy here used is identical with Bacteriumphlei or the timothy grass bacillus of Moeller and is carried in this labora-tory as Bacillus timothy 213. Samuel R. Damon 901 Examination of the growth curves seems to indicate the presencein these organisms of a growth-promoting substance that is capableof inducing rapid develo
The Journal of biological chemistry . ents were carried out,however, and the results are shown in Figs. 4 and 5. In one case5 per cent of the organisms was added to the ration and in theother 10 per cent was used to replace a like amount of starch. 2 The culture of Bacillus timothy here used is identical with Bacteriumphlei or the timothy grass bacillus of Moeller and is carried in this labora-tory as Bacillus timothy 213. Samuel R. Damon 901 Examination of the growth curves seems to indicate the presencein these organisms of a growth-promoting substance that is capableof inducing rapid development of the animals and growth seemsto be somewhat more rapid in the case of the addition of 10 percent of the bacteria. DISCUSSION. The author desires to take strong exception to the use of theterm vitamin/ as applied to any growth-stimulating substanceof bacterial origin until such substance has been subjected to the qpaTnsI65l ?PenodlCasein 18 ?PeTiodZ, 18 Suqo n n Starch 51 *l Salt Mixture 185 3 3 BuTTeT 10 10 B tiTTiaihu 10. Fig. 5. 10 per cent of Bacillus timothij 213 added at the point indicated bythe cross, seemed to stimulate the animals to practically normal growth. biological test. In the work reported in this paper certainorganisms to which growiih-stimulating properties have beenattributed have been subjected to such a test with widely diver-gent results. In the case of Friedlanders bacillus and Pfeiffers,bacillus, both members of the same group of organisms, noexplanation of the discordant results presents itself. Up to thispoint the evidence for an accessory substance in bacteria wouldseem to be negative and it is not until we examine the results offeeding experiments with Bacillus timothy 213 that there appearsany suggestive evidence on the other side of the question. In 902 Growth-Promoting Substances this experiment there really appears to have been a substancesuppKed by the bacteria that satisfied the deficiency of vitaminB in the diet. We realize, however,
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