. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 164 P. F. SCHOLANDER, C. L. CLAFF AND S. L. SVEINSSON which is the well-known formula for calculating the flask constant in a Warburg manometric apparatus. In a system where CO2 is not separately absorbed, as in our system, an ex- pression for the oxygen consumption AO2 in terms of the pressure reading AP is given by the following equations: (4) (5) \ ' VJU / Assuming RQ \J2 A)2» \ / AP = APo2 - APCo2. (7) Solved for A Fo2 the final equation gives the oxygen consumption:. = AP X F. (8) 273 273 — + VLa0z VB -y It will be note
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 164 P. F. SCHOLANDER, C. L. CLAFF AND S. L. SVEINSSON which is the well-known formula for calculating the flask constant in a Warburg manometric apparatus. In a system where CO2 is not separately absorbed, as in our system, an ex- pression for the oxygen consumption AO2 in terms of the pressure reading AP is given by the following equations: (4) (5) \ ' VJU / Assuming RQ \J2 A)2» \ / AP = APo2 - APCo2. (7) Solved for A Fo2 the final equation gives the oxygen consumption:. = AP X F. (8) 273 273 — + VLa0z VB -y It will be noted that pressures which remain constant, such as those con- tributed by water vapor, nitrogen and surface tension of the bubble, cancel out in equations 1 and 2 and are of no influence as long as they remain constant. (See derivation of equation 3 in Dixon, 1943.) Besides the pressure difference AP read on the manometer, we must know T, VB, VL, ao2, and aco2- T is equal to the water bath temperature. VB is very small and can be estimated closely enough from its measurement in the ocular eyepiece. It can be better estimated by knowing the volume per milli- meter of the diver filament and calculating the volume of the diver from its measured length. With a specific gravity of Saran of , the bubble volume will be the diver volume times VL is obtained from measuring in the binocular the diameter and the height of the chamber, allowing for the rounded upper corners. In order to save re- peated calculations a chart is prepared, where on a log log paper the diameter from mm. is plotted against the volume per millimeter height, which at mm. diameter is 314,000 MM! and at is 503,000 w\. A straight line is drawn through these points. The volume/mm, read on the graph multiplied by the height in millimeters gives the chamber volume in /i/zl. The cell may con- veniently and without significant error be considered water. The diver volume plus the bubble volume is usua
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
