. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 120 T. BOLLNER ET AL ulin adsorbed to 5 nm colloidal gold. Janssen. Belgium) diluted 1:80 in the same buffer. Finally the sections were washed with the same medium as after primary serum, rinsed in distilled water several times (5 min each at least), developed in 100 ^1 silver enhancer kit (Janssen), washed in water, and coverslipped. Indirect immunofluorescense as well as the peroxidase- antiperoxidase (PAP) method of Sternberger (1979) were also tried; the same primary antibodies were visualized by either a second layer of
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 120 T. BOLLNER ET AL ulin adsorbed to 5 nm colloidal gold. Janssen. Belgium) diluted 1:80 in the same buffer. Finally the sections were washed with the same medium as after primary serum, rinsed in distilled water several times (5 min each at least), developed in 100 ^1 silver enhancer kit (Janssen), washed in water, and coverslipped. Indirect immunofluorescense as well as the peroxidase- antiperoxidase (PAP) method of Sternberger (1979) were also tried; the same primary antibodies were visualized by either a second layer of FITC-conjugated sheep anti- rabbit serum (SIGMA), or by unlabeled sheep anti-rabbit serum (Statens Bakteriologiska Laboratorium, Stockholm) followed by PAP complex (Dakopatts). The antiserum against glutaraldehyde-conjugated 7- aminobutyric acid (GABA antiserum 26) was raised, pu- rified, and characterized as described previously (Ottersen and Storm-Mathisen, 1984b; Ottersen el ai. 1986). For all methods used, the controls included absorption of GABA antiserum with GABA-glutaraldehyde complexes (GABA-G) and glutamate complexes (Glu-G) at final concentrations of 300 ^Af. or replacement of the primary antiserum with normal rabbit serum. Furthermore, the immunoreactivity of the antiserum used was tested ac- cording to the filter disc method described by Ottersen and Storm-Mathisen (1984b). The fixation conjugates spotted on the discs were made from macromolecules ex- tracted from rat brain homogenate and from homogenate of the neural complex from the ascidian Ciona intestinalis. Results The central nervous system of Oikopleiira dioica con- sists of an anterior ganglion (brain) and tail ganglia. The anterior part of the brain is extended into paired bulbs, and in the mid-region it has a sensory vesicle. The brain is connected to several ganglia in the tail by a solid nerve cord. The largest of these tail ganglia is referred to as the caudal ganglion (Figs. 1, 2). Although the brai
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
