. A manual of bacteriology. Bacteriology. EXAMINATION OF BACTERIA WITH THE MICROSCOPE. 27 should be employed, but none of these appear exact, and one soon acquires by practice an idea as to how long the manip- ulation should take. Indeed, this varies with the character of the preparation. The heat of the flame serves to dry the bacteria upon the cover-glass and make them adhere per- manently in position. It also aids in the penetration of the staining dyes; but it is not sufficient, when applied" in this mahner, to kill all kinds of bacteria, especially those containing spores. After it h
. A manual of bacteriology. Bacteriology. EXAMINATION OF BACTERIA WITH THE MICROSCOPE. 27 should be employed, but none of these appear exact, and one soon acquires by practice an idea as to how long the manip- ulation should take. Indeed, this varies with the character of the preparation. The heat of the flame serves to dry the bacteria upon the cover-glass and make them adhere per- manently in position. It also aids in the penetration of the staining dyes; but it is not sufficient, when applied" in this mahner, to kill all kinds of bacteria, especially those containing spores. After it has been passed through the flame three times, the preparation may be stained with a solution of one of the aniline dyes, as described below, and after washing in water and drying may be mounted, face down, in Canada balsam. Fig. g.—Kirkbride forceps for holding slides. upon a glass slide. It makes a suitable object to be examined with the oil-immersion objective. The smear preparation may equally well be made directly upon the glass slide. The fixation in the flame must then occupy a longer time than with the small and thin cover-glass. Such preparations have the advantage that several may be made upon one slide, and that after staining them they may be examined in cedar-oil, with the oil-immersion lens, without the use of the cover-glass and Canada balsam. The forceps of Kirkbride will be found convenient for staining on the slide. Experiments performed in the writer's laboratory have shown that the ordinary method of fixation in the flame, when ap- plied to bacteria spread upon slides, has little effect on the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Williams, Herbert Upham, 1866-; Bolton, Benjamin Meade, 1857-1929. Philadelphia, P. Blakiston's Son & Co.
Size: 3243px × 771px
Photo credit: © The Book Worm / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1900, booksubjectbacteri, bookyear1908
