. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. IMMUNOCHEMICAL DETECTION OF PREDATION 503 eoa -^ 500- 400- § 300- !§ 5 200- •fc 100- co. 100% v T3 C 80% O -D CO 60% O w 40% £ _ro h20% S? 20 40 60 0% 100 120 140 160 180 -1, Strombidium (cells ml ) Figure 4. Ingestion rate (I) of first-feeding anchovy larvae as a function of concentration of the ciliate Strombidium sp. (C), determined by prey disappearance experiments. The right hand ordinate indicates daily car- bon-specific ingestion rates, assuming feeding for 12 h each day. The ingestion rate is described by: *C 54
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. IMMUNOCHEMICAL DETECTION OF PREDATION 503 eoa -^ 500- 400- § 300- !§ 5 200- •fc 100- co. 100% v T3 C 80% O -D CO 60% O w 40% £ _ro h20% S? 20 40 60 0% 100 120 140 160 180 -1, Strombidium (cells ml ) Figure 4. Ingestion rate (I) of first-feeding anchovy larvae as a function of concentration of the ciliate Strombidium sp. (C), determined by prey disappearance experiments. The right hand ordinate indicates daily car- bon-specific ingestion rates, assuming feeding for 12 h each day. The ingestion rate is described by: *C + C This curve was fitted by nonlinear least squares using the Simplex al- gorithm. Monte Carlo simulations (n = 1000) based on standard devia- tions of replicate cell counts indicated that the coefficient of variation about individual ingestion rates was approximately 15%. Seven to twenty-five first-feeding larvae were incubated for 6-8 h with ciliates in illuminated 800-ml containers. Aliquots were preserved in 2% acid Lugols solution, then four subsamples settled overnight by the Uter- mohl procedure and ciliates enumerated by inverted microscope. Control containers lacking larvae were run at each experimental density (average ciliate recovery 102%). Predation rates were calculated from the equations in (28). (2) implies that if differential digestion of antigenic and non-antigenic protein occurs, the time course of this re- lationship must be determined. The antigenic properties of the prey ingested by a penaeid shrimp did not change over time during digestion (18), although this was not the case with a carid shrimp ingesting different prey (20). The immunochemical detectability of ingested prey varied with location in the intestinal tract of Octopus vulgaris (19). However, because first-feeding larval anchovy have a relatively simple tubular gut, we do not expect digestion to be compartmentalized. As an alternate solution to the issue of differential digestion, we
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
