. Electron microscopy; proceedings of the Stockholm Conference, September, 1956. Electron microscopy. a ** T^ ,' -.»'â Fig. 1. Striated muscle tissue of the axo\otl(Amblystoma pitnctotttm). The section has been treated with 4% acetic acid. tails of the structure of the tissues less visible. There- fore, it is recommended that before enclosing the objects in gelatin, fixation should be shorter and washing longer. It can be supposed, however, that the presence of the background obliterating the bound- aries of some structures, is connected with the presence of substances which are otherwise eli
. Electron microscopy; proceedings of the Stockholm Conference, September, 1956. Electron microscopy. a ** T^ ,' -.»'â Fig. 1. Striated muscle tissue of the axo\otl(Amblystoma pitnctotttm). The section has been treated with 4% acetic acid. tails of the structure of the tissues less visible. There- fore, it is recommended that before enclosing the objects in gelatin, fixation should be shorter and washing longer. It can be supposed, however, that the presence of the background obliterating the bound- aries of some structures, is connected with the presence of substances which are otherwise elimi- nated or precipitated, while the specimen is treated with alcohols and methacrylates. In the case of thinner sections it is recom- mended to examine them without removal of em- bedding medium (gelatin). In this case the sections should be expanded in cold water (about - I to ^ rc). The tissues, embedded in gelatin, can be easily cut with a glass knife for sections 1 fi thick for exa- mination in the light microscope. In this case the blocks should not be too dry and, therefore, are kept in open vessels. The sections are glued up to micro- scope slides with albumen, to which some glycerine has been added. The gelatin is dissolved in warm water. References 1. Brandes, C. H., Mikrokosmos 44, N7, 167 (1955). 2. FiRMiNGER, H. I., Stain Techno/. 25, N 3, 121 (1950). 3. Newman, S. B., Borysko, E., and Swerdlow, M., /. Research Nat. Bur. Standards 43, 183 (1949). 4. Palade, G. E., J. Med. 95, 285 (1952). 5. Richards, A. G., Anderson, T. F., and Hance, R. T., Proc. Soc. Biol. Med. 51, N 1, 148 (1942). ^ This method ofmounting sections was elaborated together with V. I. Birjuzova, one of the workers of our Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original European Regional Conference
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