. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ECDYSIOTROPIC ACTIVITY OF JUVENILE HORMONE 301 100 *- 80 c 0> E a. 2 o> I 60 ^40 o^ > O E 3 o 20 0. I I 46 8 10 12 Weeks After Pupation 14 16 FIGURE 1. Initiation of development by groups of short day, diapause-destined pupae receiving topi- cal application of JHA on the day of pupal ecdysis. Dosages of hormone per pupa were 200 ng (•), 20 ng (V), 2 jug (D). Hormone was delivered in 2 jil acetone and one group of pupae received topical application of 2 fil acetone only (A). Pupae were examined for tracheal apolysis
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ECDYSIOTROPIC ACTIVITY OF JUVENILE HORMONE 301 100 *- 80 c 0> E a. 2 o> I 60 ^40 o^ > O E 3 o 20 0. I I 46 8 10 12 Weeks After Pupation 14 16 FIGURE 1. Initiation of development by groups of short day, diapause-destined pupae receiving topi- cal application of JHA on the day of pupal ecdysis. Dosages of hormone per pupa were 200 ng (•), 20 ng (V), 2 jug (D). Hormone was delivered in 2 jil acetone and one group of pupae received topical application of 2 fil acetone only (A). Pupae were examined for tracheal apolysis at weekly intervals. The cumulative percentage of each group demonstrating apolysis is plotted as a function of time after pupation. The lines are drawn by inspection. Ecdysiotropic effects of JHA We next inquired whether the acceleration of development witnessed in prepara- tions treated with JHA was accompanied by an elevation of the ecdysteroid liter. Three groups were established: intact diapause-destined pupae, sham-operated dia- pause-destined pupae, and diapause-destined pupae from which the brain had been extirpated on Day 2. To individuals in each of these groups we administered daily topical applications of 20 ^g of JHA beginning on pupal Day 3 and continuing until the onset of adult development. Hemolymph was collected on the day that tracheal apolysis was first observed and assayed for ecdysteroids by RIA. Ecdysteroid levels were also measured in hemolymph from two JH A-free control groups, one consisting of 4-week-old diapausing pupae, the other of individuals that had been in diapause for at least 4 weeks and had undergone tracheal apolysis during the 24 h preceding hemolymph collection. Intact JHA-treated pupae initiated development 5-14 days after initiation of. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
