. The microscope; an introduction to microscopic methods and to histology. Microscopes. CH. VII] PARAFFIN SECTIONING hasten the solution of the paraffin by moving the slide in the solvent. In this way it may be dissolved in 5 to 10 minutes, or even less. It will do no harm to leave the slide in the benzin or xvlene over night. Two or three days even might not do any harm, but it is usually better to proceed at once to the other Fig. 160. Apparatus and regents with which the slide holders are used. With this apparatus it is easy to prepare specimens in large numbers very expeditiou
. The microscope; an introduction to microscopic methods and to histology. Microscopes. CH. VII] PARAFFIN SECTIONING hasten the solution of the paraffin by moving the slide in the solvent. In this way it may be dissolved in 5 to 10 minutes, or even less. It will do no harm to leave the slide in the benzin or xvlene over night. Two or three days even might not do any harm, but it is usually better to proceed at once to the other Fig. 160. Apparatus and regents with which the slide holders are used. With this apparatus it is easy to prepare specimens in large numbers very expeditiously. After the sections are fastened to the slide and placed in the holder, the slides need not be touched during all the operations until they are finally ready to be mounted in balsam. Each holder contains from 12 to 14 slides. (§ 277-300). The bottles for the reagents are glass stoppered specimen or museum bottles. (Mix, four. Ap. Micr. /SgS,p. 171.) Compare Fig. 14S. 'i 292. Removing the Xylene or Benzin—From the xylene or benzin plunge the slide bearing the sections into a jar of 95'',, alcohol, and leave it for a few minutes, or move it around in the alcohol for half a minute or so. \ 293. Staining the Sections with an Alcoholic Dye.—With an alcoholic stain like hydrochloric acid carmine, remove the slide from the alcohol, and add the stain directly after draining the slide, Do not allow the stain to become dry, for that would injure the tissue. Wash away the stain with 67% alcohol, then dehydrate with 95'',, alcohol, clear and mount in balsam as described below. \ 294. Staining with as Aqueous Dye.—Wash away the 95% alcohol from the slide bearing the sections by plunging it into a jar of water and moving it around a moment. Then add the stain to the sections with a pipette, or immerse the slide in a jar of the stain, and allow the stain to act from 5 to 10 minutes. Wash thoroughlv with water. \ 295. Staining with a General Dye—Counterstaining.—If it is desired
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