. Control of ovulation; proceedings of the conference held at Endicott House, Dedham, Massachusetts, 1960. Ovulation -- Regulation. 190 Discussions Dr. Roland K. Meykr: 1 shall describe data obtained in my laboratory by Mr. W. F. Strauss. The experiment was based on the publications of H. H. Cole (Am. J. Physiol. 119, 704, 1937) who reported mating and fetal development prior to parturition in immature rats treated with pregnant mare serum gonadotropin (PMS). In our experi- ment female rats of the Holt/man strain were injected subcutaneously during the morning of the 30lh day of life with one
. Control of ovulation; proceedings of the conference held at Endicott House, Dedham, Massachusetts, 1960. Ovulation -- Regulation. 190 Discussions Dr. Roland K. Meykr: 1 shall describe data obtained in my laboratory by Mr. W. F. Strauss. The experiment was based on the publications of H. H. Cole (Am. J. Physiol. 119, 704, 1937) who reported mating and fetal development prior to parturition in immature rats treated with pregnant mare serum gonadotropin (PMS). In our experi- ment female rats of the Holt/man strain were injected subcutaneously during the morning of the 30lh day of life with one single dose of Cartland-Nelson unit of Gonadogen (The Upjohn Company). Forty-eight hours later one or two females were placed with two adult males. Seventy-two hours after PMS injections, vaginal smears were taken; sperm or a vaginal plug was considered as evidence that mating had occurred. He started with 144 female rats but many of these were used for other studies as the experiment progressed. On the basis of the data obtained from this group we predict that in a comparable group of 100 rats, 86 would mate, 81 would have an average 5 100- • INDIVIDUAL ANIMALS -o-AVERASE WHEN MORE THAN ONE RAT AUTOPSIEO AT A QIVEN HOUR. HOURS AFTER OVULATOR WAS INJECTED Fig. 1. Number of ova recovered from oviducts of immature hypophysectomized rats injected subcutaneously with a FSH preparation, and intravenously with a preparation containing both FSH and LH activity. of 10 implantation sites on the 7th day after ovulation, and 75 would parturate an average of young (average gestation days). The young mothers would lactate and raise an average of young to time of weaning. Another group of immature females were treated with PMS as described for the first group, except that they were not allowed to mate. The time of the first and subsequent estrous periods was determined by use of vaginal smears. The first estrous smear after the one associated with vaginal opening occurred
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