. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 172 M. J. ELY AND A. M JUNGREIS group was then subjected to an kr X-ray exposure (325 r/minute) 24 hours following the second ecdysone administration. Day 1 animals receiving 4 ecdysone/gram wet weight without subsequent irradiation served as a control group. Secondly, day 12 animals were treated in a manner identical to that used for day 1 animals with the exception that the ED0 X-irradiation exposure was 25 kr (325 r/minute). Thirdly, day 4 animals were treated in a manner similar to day 1 animals except that at 6
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 172 M. J. ELY AND A. M JUNGREIS group was then subjected to an kr X-ray exposure (325 r/minute) 24 hours following the second ecdysone administration. Day 1 animals receiving 4 ecdysone/gram wet weight without subsequent irradiation served as a control group. Secondly, day 12 animals were treated in a manner identical to that used for day 1 animals with the exception that the ED0 X-irradiation exposure was 25 kr (325 r/minute). Thirdly, day 4 animals were treated in a manner similar to day 1 animals except that at 6, 12. 24, 36, and 48 hours post ecdysone injections the animals were irradiated at an ED0 level. Fourthly, day 4 animals were X-irradi- ated with an exposure of kr (325 r/minute) 24 hours following administration of 2. 4, 6, 12. and 16 micrograms of ecdysone per gram of wet weight. Eclosion success was determined for each experimental group and expressed as a percentage of eclosion success observed in noninjected animals irradiated at similar time intervals. Day 4 and day 12 data were analyzed using single classi- fication analysis of variance. The Student-Newman-Keuls test was employed for a comparison among means (Sokal and Rohlf, 1969). RESULTS Effects of X-irradiation on eclosion The effect of X-irradiation on eclosion of M. during the pupal-adult trans- formation was investigated by initially determining the ED50 for animals on each day of development and by utilizing the ED50 values as a relative measure of radiation sensitivity (Fig. 1). An initial period of nearly constant radiosensitivity 80- z o u uj 40' Ul a. i Z O 20 -o—0_o—o—Q. I I I I ' 12 16 20 DAYS AFTER LARVAL-PUPAL ECDYSIS FIGURE 1. ED50 values (X-ray exposures required to prevent eclosion in 50% of the irradiated animals) during pupal-adult development of Manduca Please note that these images are extracted from scanned page images that may have been digitally enhanced for readabilit
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
