. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ENZYMIC HISTOCHEMISTRY OF GRANULAR COMPONENTS IN DIGESTIVE GLAND CELLS OF THE ROMAN SNAIL, HELIX POMATIA1 ROBERT M. ROSENBAUM AND BRUCE DITZION Department of Pathology, Albert Einstein College of Medicine, Nezv York 61, New York The digestive gland of Helix pomatia has been studied histologically especially during feeding and digestion (Krijgsman, 1925, 1929; Thiele, 1953; Guardabassi and Ferreri, 1953 among others). In addition, biochemical studies employing homogenates of gland tissue or aliquots of crop or digestive fluid
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ENZYMIC HISTOCHEMISTRY OF GRANULAR COMPONENTS IN DIGESTIVE GLAND CELLS OF THE ROMAN SNAIL, HELIX POMATIA1 ROBERT M. ROSENBAUM AND BRUCE DITZION Department of Pathology, Albert Einstein College of Medicine, Nezv York 61, New York The digestive gland of Helix pomatia has been studied histologically especially during feeding and digestion (Krijgsman, 1925, 1929; Thiele, 1953; Guardabassi and Ferreri, 1953 among others). In addition, biochemical studies employing homogenates of gland tissue or aliquots of crop or digestive fluid have established that both intra- and extracellular hydrolytic enzymes are associated with the gland tissue (Holden and Tracey, 1950). Such methods, while providing quantitative data, do not permit direct study of the intracellular enzymic activity within the gland. Helix appears able to produce enormous amounts of several hydrolytic enzymes rapidly (Holden and Tracey, 1950; Billett, 1954; Dodgson and Powell, 1959) and it is therefore not unreasonable to assume that a highly developed synthetic machinery for secretion of extracellular hydrolases may exist within the cells concerned. The activity of several hydrolytic enzymes, including acid phosphatase, /?-glu- curonidase, and several "cathepsins," has been shown by biochemical methods to vary according to the feeding cycle (Holden and Tracey, 1950; Jarrige and Henry, 1952). Several histochemical studies have shown /?-glucuronidase activity (Billett and McGee-Russell, 1955) and acid and alkaline phosphatase activity (Guardabassi and Ferreri, 1953; Nakazima, 1956) within digestive gland tubules. To date, no reports have dealt with visualization of enzymic activity within specific digestive gland cells. In this investigation we were concerned not only with achieving intracellular localization of specific hydrolases, but also with a comparison of intracellular en- zymic activity during periods of starvation and active f
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