. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. ties on a squareboard, with the abdomen upward. 3. Note the presence of any external lesions, such asswellings, ulcerations, etc. 4. Wash with a solution of mercuric chlorid (i: 1,000)followed by alcohol. 5. Lift the skin over the pubes with the forceps, andwith the scissors make an incision along the median linewell above the sternal notch; then diagonal incisionsextending along the fore- and hind-legs. 6. Cut the skin away with a moderately sharpknife, avoiding opening the abdominal cavity. 7. Open th
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. ties on a squareboard, with the abdomen upward. 3. Note the presence of any external lesions, such asswellings, ulcerations, etc. 4. Wash with a solution of mercuric chlorid (i: 1,000)followed by alcohol. 5. Lift the skin over the pubes with the forceps, andwith the scissors make an incision along the median linewell above the sternal notch; then diagonal incisionsextending along the fore- and hind-legs. 6. Cut the skin away with a moderately sharpknife, avoiding opening the abdominal cavity. 7. Open the abdomen by a median incision from theliubes to the sternum. 8. Remove the anterior thoracic wall by cuttingaway the ribs from below upward on each side to thethoracic apex. The viscera are now exposed. Cultures and smearsshould be made from the hearts blood, peritonealcavity, spleen, liver, and localized foci of suppuration. Gram stains are of special value inasmuch as staphy-lococci are gram positive, while the tissues are moreor less decolorized. I08 LABORATORY GUIDE IN BACTERIOLOGY. KIDNEY-— ADHENALGLANDS INGUINALGLANDS OcP-MJ-NGUINALGLANDS Fig. 31Autopsy of a Guinea-Pig(Diagrammatic) IMPORTANT PATHOGENIC BACTERIA 109 EXERCISE 2. THE PYOGENIC GROUP (SUBGROUP b)Members— Streptococcus pneumoniae. Micrococcus zymogenes. Micrococcus gonorrheas. M. gonorrheae is difficult to cultivate on labora-tory media. It is a parasite and requires special mediafor cultivation. For these reasons it is sufficient tostudy the characteristic morphology in smears madefrom gonorrheal pus. Methylene blue or Pappenheimsstain (p. 4s) and Gram stains should be made. Inoculate agar slants from laboratory cultures ofStr. pnetunoniae and M. (M. zymogenes)— MacCaUum and Hastings, Jour. Exper. Med., 1899, 4, p. 521. Harris and Longcope, CentralU. /. Bakt.,~ 1901, 30, ; P- 3S3 (printed in English). Birge, Johns Hopkins Hospital Bull., 1905, 16, p. 309. 1. Routine study.—N
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