Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . at ammonia may be split off from urea and thus give figureswhich are somewhat high. It has been found that if the apparatus be keptat 38° C, the time necessary for this reaction may be reduced to 48 we add to the urine instead of the milk of lime, gram of sodium carbonate THE URINE. 217 and about 10 grams of sodium chlorid, no ammonia will be split off from theurea and no decomposition of the urine will occur (Schaffer).Folins Method. The ammonia in this method^ is set fr
Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . at ammonia may be split off from urea and thus give figureswhich are somewhat high. It has been found that if the apparatus be keptat 38° C, the time necessary for this reaction may be reduced to 48 we add to the urine instead of the milk of lime, gram of sodium carbonate THE URINE. 217 and about 10 grams of sodium chlorid, no ammonia will be split off from theurea and no decomposition of the urine will occur (Schaffer).Folins Method. The ammonia in this method^ is set free by the addition of a weak alkali(sodium carbonate), is then removed from the urine at ordinary room tem-perature by means of a strong air-current, is collected in tenth-normal sulphuricacid and then titrated. Technic. Twenty-five of urine are measured into an aerometer cylinder (30to 40 cm. high), and about a gram of dry sodium carbonate and some crudepetroleum (to prevent foaming) are added. The upper end of the cylinderis then closed by means of a doubly perforated rubber stopper, through which. Fig. 76.—Folins ammonia apparatus. {Hawk.) pass two glass tubes, only one of which is long enough to reach below thesurface of the liquid. The shorter tube (about 10 cm.) in length is connectedwith a calcium chlorid tube filled with cotton, which in turn is connectedwith a glass tube extending to the bottom of a wide-mouthed bottle (capacityabout 500 ) which contains 20 of tenth-normal sulphuric acid, 200 water and a few drops of an indicator (ahzarin-red). The complete ab-sorption of -the ammonia by the sulphuric acid is most easily insured by theuse of a simple absorption tube which compels a very intimate contact of theair coming from the cylinder with the acid and water in the absorption absorption bulb consists of a glass tube, measuring about 8 mm., indiameter, one extremity of which has been blown into a small bulb. Bymeans of a heated pla
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