Archive image from page 44 of The development of a cosmid. The development of a cosmid map of chromosome 12p13 . developmentofcos00belk Year: 1998 dUT DIG PCR Reaction DIG labeledMarker :? Marker Dot Blot o o o o o o o o o o DIG labeledMarker 5 r Corrjjcl Di J A cJjJutJCjrj: Figure 10. Digoxigenin Hybridization of Target STS marker to Cosmid DNA Dot Blot. The gray represents cosmid DNA which has bound the DIG labeled marker DNA. AGAROSE Gel Electrophoresis. Agarose gel electrophoresis was employed to determine the relationship among positive cosmids from hybridization and to analyz
Archive image from page 44 of The development of a cosmid. The development of a cosmid map of chromosome 12p13 . developmentofcos00belk Year: 1998 dUT DIG PCR Reaction DIG labeledMarker :? Marker Dot Blot o o o o o o o o o o DIG labeledMarker 5 r Corrjjcl Di J A cJjJutJCjrj: Figure 10. Digoxigenin Hybridization of Target STS marker to Cosmid DNA Dot Blot. The gray represents cosmid DNA which has bound the DIG labeled marker DNA. AGAROSE Gel Electrophoresis. Agarose gel electrophoresis was employed to determine the relationship among positive cosmids from hybridization and to analyze PCR results. Agarose gel electrophoresis is the standard method of separating DNA of lengths from 200 base pairs to 50 kilobases for identification and purification procedures.' A rectangular plate of agarose gel is subjected to a constant voltage and the DNA loaded into the gel. obtained by PCR or other means, migrates toward the positive electrode due to its negative charge (Figure 11 .a). When a gel is run with a DNA ladder of knovm size it is easy to determine the sizes of the DNA that are separated across the gel in the procedure (FIGURE 11 .b). By plotting the migration distances of known fragments as well as unknown fragments versus the sizes of the known fragments on a logarithmic plot one can 21
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