. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 242 L. S. QUACKENBUSH AND M. FINGERMAN Uca E ry t h roptiores 5 Volts T " i" • r T. 30 60 90 120 Time in Minutes FIGURE 3. Mean chromatophore stage of isolated legs given perfusates from isolated eyestalks stimulated with 5 volts or 10 volts (solid squares) compared to isolated legs given perfusates from unstimulated eyestalks (open squares). Solid lines connect the values from isolated legs with fully dispersed red pigment. Isolated legs with initially fully concentrated red pigment were also tested, but showed no
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 242 L. S. QUACKENBUSH AND M. FINGERMAN Uca E ry t h roptiores 5 Volts T " i" • r T. 30 60 90 120 Time in Minutes FIGURE 3. Mean chromatophore stage of isolated legs given perfusates from isolated eyestalks stimulated with 5 volts or 10 volts (solid squares) compared to isolated legs given perfusates from unstimulated eyestalks (open squares). Solid lines connect the values from isolated legs with fully dispersed red pigment. Isolated legs with initially fully concentrated red pigment were also tested, but showed no effect of perfusate injection (data not shown). All values are means ± one standard deviation (n = 21, seven replicates). ± ; and ± ). Two different fractions had peptides ( ± pg protein for each fraction) in the low molecular weight range (less than 5,000 d) that only affected black chromatophores. BPDH activity was resolved into 3 fractions after the void volume (Rf ± had ± pg protein, ± and ± each had ± pg protein). It should be noted that two fractions containing BPDH had no BPCH activity. The presence of several sizes of peptides which have biological activity in these perfusates suggests that processing of precursors may occur within the eyestalk complex. Furthermore, the isolated eyestalk complex releases these large sized compounds when stimulated with voltage. Finally, the eyestalk complex contains large compounds which can affect two different target cells, as well as different compounds which have activity on only one target cell. Isolated eyestalks stimulated with NE Isolated eyestalks were bathed in saline to which one of six different NE concen- trations was added (10~"-10~6 M) (Fig. 5). Exogenous NE did not induce the release of any substance which produced either significant dispersion or concentration of the red pigment. Isolated eyestalks in saline with 10" MNE relea
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
