. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 256 R. A FLUCK ET Figure 1. Diffusion of fluorescein around the medaka egg. The approximate position of the injection site is marked with an arrowhead. (A) By 5 min after injection of the dye. it had spread more than 50° arc in all directions around the egg. (B) By 18 min, it was present about 90° arc from the injection site. (C) At 74 min, it had spread to the antipode of the injection site. Scale bar, 250 ^m. formed to the shape of the micropipette. When fluid was injected into the ooplasm, it distended the yolk membr
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 256 R. A FLUCK ET Figure 1. Diffusion of fluorescein around the medaka egg. The approximate position of the injection site is marked with an arrowhead. (A) By 5 min after injection of the dye. it had spread more than 50° arc in all directions around the egg. (B) By 18 min, it was present about 90° arc from the injection site. (C) At 74 min, it had spread to the antipode of the injection site. Scale bar, 250 ^m. formed to the shape of the micropipette. When fluid was injected into the ooplasm, it distended the yolk membrane near the tip of the micropipette, thus visually confirming that fluid had been injected into the ooplasm and not the yolk vacuole. As the pipette was slowly withdrawn, the yolk membrane returned to its normal position, causing the injectate to be spread quickly over a large area of the ooplasm. Fluid was injected either equatorially (within 30° arc of the equator) or near the vegetal pole (within 30° arc of the vegetal pole). Dibromo-BAPTA was injected into the yolk vacuole of four eggs. Such eggs were penetrated with a micropipette in the usual manner, except that the tip of the pipette also penetrated the yolk membrane. The injectate thus did not distend the yolk membrane but did transiently change the optical properties of the yolk near the tip of the pipette, confirming that fluid had been injected. We injected dibromo-BAPTA into eggs in three series of experiments: I (winter 1990, in which we successfully injected butter into the ooplasm of 45 eggs from 12 fe- males), II (winter 1992, 27 eggs from 4 females), and III (summer 1992, 20 eggs from 4 females). In addition, we injected solutions of KiSOj or KC1 into 15 eggs; injected dimethyl-BAPTA into 10 eggs from two females; injected dibromo-BAPTA into the yolk vacuole of 4 eggs; and monitored the development of 44 uninjected control eggs. Observation of the effects of buffer injection Ooplasmic movements in at least
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
