. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 52 T. G. WOLCOTT AND A. H. MINES. ^^ " Figure 2. Completed transmitters. Above: encapsulated with wax. Below: encapsulated in vinyl sleeving. Hooked wire at right plugs the oil-fill hole. the package backfilled with mineral oil until all air hub- bies were eliminated (Fig. 2). After some practice, we could prepare a crab, insert the electrodes, and attach the transmitter to the crab in about 20 min. We determined by dissections that, in the blue crab, the muscles that close the mandibles originate on the front of the ex
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 52 T. G. WOLCOTT AND A. H. MINES. ^^ " Figure 2. Completed transmitters. Above: encapsulated with wax. Below: encapsulated in vinyl sleeving. Hooked wire at right plugs the oil-fill hole. the package backfilled with mineral oil until all air hub- bies were eliminated (Fig. 2). After some practice, we could prepare a crab, insert the electrodes, and attach the transmitter to the crab in about 20 min. We determined by dissections that, in the blue crab, the muscles that close the mandibles originate on the front of the exoskeleton, ventro-lateral to the eye sockets (Fig. 3), where electrodes could be inserted con- veniently into the end of the muscle and immobilized by fastening to the exoskeleton. Reference electrodes were sited about 1 cm laterally to enter the hemocoel away from any potentially interfering muscles. The surface of the exoskeleton at the electrode sites was cleaned and dried by swabbing with acetone. Using a small ball- tipped dental bit (no. 1/2). holes were drilled nearly through the calcified layer of the exoskeleton at the de- sired locations, with care not to drill completely through the exoskeleton. A patch of thin rubber (, heavy den- tal dam) was glued to the area using a high-performance cyanoacrylate glue (Dexter Hysol 2-C-500) to provide a septum through which electrodes could be inserted with- out loss of blood. (Bleeding interferes with glue bonding in addition to causing undesirable physiological effects.) Electrodes were fabricated of number 0 stainless steel insect pins cut into 1 cm lengths. The electrodes were bent so that they penetrated about 3 mm inside the exo- skeleton. The active (muscle) electrode was angled to lie parallel to the muscle fibers when the outside portion was cemented flat to the exoskeleton. The reference electrode was bent about 90 degrees to enter the hemocoel perpen- dicular to the skeletal surface. The distal ends of the stainles
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
