. Bulletin of the Museum of Comparative Zoology at Harvard College. Zoology. Trade-Off Responses to Loading in the Mammalian Limb • Liebennan and Pearson 273 •a o 2 Radius of Gyration Proximal ' Distal. Radius of Gyration Proximal ' Distal Figure 2. Predicted trade-off between periosteal growth (modeling) and Haversian remodeling (repair) rates in cortical bone as a function of the radius of gyration. and water ad libitum. Fluorescent dyes that incorporate into bone mineral were administered by intraperitoneal injection at the start of the experiment (calcein, 20 mg/kg), on day 30 (oxytetracyl
. Bulletin of the Museum of Comparative Zoology at Harvard College. Zoology. Trade-Off Responses to Loading in the Mammalian Limb • Liebennan and Pearson 273 •a o 2 Radius of Gyration Proximal ' Distal. Radius of Gyration Proximal ' Distal Figure 2. Predicted trade-off between periosteal growth (modeling) and Haversian remodeling (repair) rates in cortical bone as a function of the radius of gyration. and water ad libitum. Fluorescent dyes that incorporate into bone mineral were administered by intraperitoneal injection at the start of the experiment (calcein, 20 mg/kg), on day 30 (oxytetracyline, 50 mg/ kg), and on day 63 (xylenol orange, 25 mg/ kg). Body mass was measured weekly. At the end of the experiment, total limb length from the greater trochanter of the femur to the ground was measured for each subject. The subjects were then eu- thanized, and their limbs bones were re- moved and defleshed. The radii of gyration from the femoro-acetabular joint to the midshaft of the femur, tibia, and metatar- sal were measured at simulated midstance using a plastic tape measure (accurate to 1 mm) on each animal after death. Articular lengths of the femur, tibia, and metatarsal were measured using digital calipers (ac- curate to mm). Femoral length was measured from the most proximal point on the femoral head to the line connecting the two distal condyles. Tibial length was measured from the center of the lateral condylar surface to the center of the distal articular surface. Metatarsal length was measured from the center of the proximal articular surface to the most distal point of the distal articular surface. Cross-Sectional Geometry and Histologic Analysis A 2-cm section was cut from the limb midshaft of each left femur, tibia, and metatarsal, and the inarrow was removed. After measuring their densities, sections were fixed in 100% ethanol, and then si- multaneously stained and dehydrated in a solution of 1% basic fuchsin in denatured ethanol under 20 mm Hg va
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