. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 220 The Cultivation of Anaerobic Organisms his recommendation having recently been revived by Park and made successful for the cultivation of the tetanus bacillus. The paraffin floats upon the surface of the medium, melts during sterilization but does not mix with it, and "sets" when cool. The inoculation is to be made while the culture medium is warm, after boiling and before the parafSn sets. Koch studied the colonies of anaerobic organisms b
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 220 The Cultivation of Anaerobic Organisms his recommendation having recently been revived by Park and made successful for the cultivation of the tetanus bacillus. The paraffin floats upon the surface of the medium, melts during sterilization but does not mix with it, and "sets" when cool. The inoculation is to be made while the culture medium is warm, after boiling and before the parafSn sets. Koch studied the colonies of anaerobic organisms by cultivating them upon a film of gelatin covered by a thin sheet of sterilized mica, by which the air was excluded. Salamonsen has made use of apipetfor making anaerobic cultures. It is made of a glass tube a few millimeters in diam- eter, drawn out to a point at each end. The inoculated gelatin or agar-agar is drawn in while liquefied and the ends sealed. The tube, of course, contains no air, and perfect anaerobiosis results. Theobald Smith has found the fer- mentation-tube and various modifications of it excellently well adapted to the growth of anaerobes, which, of course, grow only in the closed limb. Hens' eggs have been used for anaerobic cultures, and in them the tetanus bacillus grows remarkably well. Conditions of anaerobiosis are, however, not perfect, as can be shown by the behavior of the egg itself. If oxygen be completely shut out by oiling or varnishing the shell, a fertile egg will not develop. A quite satisfactory and simple device for routine work with anaerobic organisms has been invented by Wright.* The es- sential feature consists of a pipet, D, with Figs 69, 70—Wright's a rubber tube, E, at the end, and one in- method of making anaero- . ' ' , , ,' , ,-, bic cultures in fluid media terruption connected by a rubber tube, C. (Mallory and Wright). The device will be made clear at once by a glance at the accompanying illustration. The method of employment i
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1916
