. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MOLECULAR BIOLOGY OF MOUSE EMBRYOS 293 Meiotic Maturation Oocyte J Growth N Primordial Germ Cells. MET n ) Ovulation and Fertilization (12 HOURS POST-HCG) 1-cell Stage (Day 1) 2-cell Stage (Day 2) 8-cell Stage, Compaction, Embryo Enters Uterus (Day 3) Blastocyst Growth and Expansion (Day 4) Implantation (Day 5) TROPHECTODERM FIGURE I. Diagrammatic outline of early development in the mouse. Abbreviations are as follows: PMSG, pregnant mare serum gonadotrophin; HCG, human chorionic gonadotrophin; GVBD, germinal vesicle breakdo
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MOLECULAR BIOLOGY OF MOUSE EMBRYOS 293 Meiotic Maturation Oocyte J Growth N Primordial Germ Cells. MET n ) Ovulation and Fertilization (12 HOURS POST-HCG) 1-cell Stage (Day 1) 2-cell Stage (Day 2) 8-cell Stage, Compaction, Embryo Enters Uterus (Day 3) Blastocyst Growth and Expansion (Day 4) Implantation (Day 5) TROPHECTODERM FIGURE I. Diagrammatic outline of early development in the mouse. Abbreviations are as follows: PMSG, pregnant mare serum gonadotrophin; HCG, human chorionic gonadotrophin; GVBD, germinal vesicle breakdown; MET I, first meiotic metaphase; MET II, second meiotic metaphase; ICM, inner cell mass. the fimbrial end of the oviduct occurs approximately 12 hours after HCG administra- tion (Fig. 1). The light-dark sequence is a major modulator in polyestrous mammals like mice. Endogenous LH surges lead to an ovulation time about 3 h after the mid-point of the dark period (Whitten and Champlin, 1978). Hence, it is common to employ a system in which the mouse room is maintained with 12 to 14 hours of light and 10 to 12 hours of dark with lights on at 5:00 to 7:00 hours and lights off at 19:00 hours. Injec- tions of PMSG are often scheduled between 16:00 to 20:00 hours, and HCG is admin-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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