. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 1200 Days MF(ug/ml) Figure 3. Average carapace length of Macrobrachium roxenbergii larvae during 12 days of feeding on Anemia enriched with different concentrations of methyl farnesoate. Each bar represents mean ± SE (n = 151. The results presented are taken from one representative experi- ment out of the three identical experiments performed. Using the Ar- lemia vector, approximately of the MF added to the Anemia enrichment medium (referred to as MF (^g/ml)) was found in M. rosen- bergii larva feeding for 8 h on the
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 1200 Days MF(ug/ml) Figure 3. Average carapace length of Macrobrachium roxenbergii larvae during 12 days of feeding on Anemia enriched with different concentrations of methyl farnesoate. Each bar represents mean ± SE (n = 151. The results presented are taken from one representative experi- ment out of the three identical experiments performed. Using the Ar- lemia vector, approximately of the MF added to the Anemia enrichment medium (referred to as MF (^g/ml)) was found in M. rosen- bergii larva feeding for 8 h on the enriched Anemia (Abdu el al., 1498). This suggests that the level of MF in the present study did not exceed ng MF per larva exposed to the highest dose and ng MF per larva exposed to the lowest dose. Since the average weight of a larva is about g, these levels could be expressed as ng MF/g. The calculated level for the low MF exposure is in the range of physiological level found in M. rosenbergii larvae (up to 25 ng/g, data not shownl and adults (up to 40 ng/ml, Sagi et al., 1991). Growth retardation in larvae fed on Anemia enriched with the median dose of MF was significant (P < ) only on day 12. Growth of larvae fed on Anemia that were enriched with the highest concentration of MF was markedly slower than that of the control group, the difference being significant (P < ) from day 5 onward. representative HPLC chromatogram of one such hexane extract of larvae shows the presence of the biological all- trans MF, identified by comparing its retention time with that of the external standard (data not shown) and that of the internal standard (Fig. 1). Gas chromatography-mass spectrometry of the putative peaks from larval stages 4, 6, 9, 10, and 11 showed a typical methyl farnesoate profile exhibiting selected ion monitoring at m/z 69, 114, and 121 (Fig. 2). Effect of methyl farnesoate on late larval growth and development Survival was sim
Size: 1640px × 1524px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
