. A manual of bacteriology. Bacteriology. 96 MANUAL OF BACTERIOLOGY. the medium up into foam, and also avoid wetting the plug. Carry three loopfuls from tube No. 2 into tube No. 3 in the same manner. The original material will obviously be diluted in tube No. i, more in tube No. 2 and still more in tube No. 3. The most convenient form of plate is that known as a Petri dish, a small glass dish about 8 cm. in diameter and cm. in height, provided with a cover which is a little larger, but of the same form. This dish should be cleaned and sterilized in a hot- air sterilizer at 150° C. or highe
. A manual of bacteriology. Bacteriology. 96 MANUAL OF BACTERIOLOGY. the medium up into foam, and also avoid wetting the plug. Carry three loopfuls from tube No. 2 into tube No. 3 in the same manner. The original material will obviously be diluted in tube No. i, more in tube No. 2 and still more in tube No. 3. The most convenient form of plate is that known as a Petri dish, a small glass dish about 8 cm. in diameter and cm. in height, provided with a cover which is a little larger, but of the same form. This dish should be cleaned and sterilized in a hot- air sterilizer at 150° C. or higher for an hour. When it is cool it may be used. Such dishes having previously been prepared, the contents of tube No. I are poured into one dish, and those of tube No. 2 into another and"those of tube No. 3 into a third. They are. Fig. 32.—Petri dish. to be labeled Nos. i, 2 and 3.* In pouring proceed as follows: Remove the plug of tube No. i; heat the neck of the tube in the flame; allow it to cool, holding it in a nearly horizontal position. When the tube has cooled, lift the cover of the Petri dish a little, holding it over the dish; pour the contents of tube No. i into the dish and replace the cover of the dish. The interior of the dish should be exposed as little and as short a time as possible. Tubes Nos. 2 and 3 are to be treated in the same manner. Burn the plugs, and fill the empty tubes with 5 per cent, solution of carbolic acid. They should be sterilized for an hour in the steam sterilizer on each of three days. *The labels should be moistened with the finger, which has been dipped in water. They should not be licked with the tongue. While working in the bac- teriological laboratory it is best to make it a rule that no object is to be put in the Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the origi
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectbacteri, bookyear1908
