. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 478 M. J. SNYDER AND E. S. CHANG T3 O LJ -C a ^v o E 0) o •*-* c tu Q. 100n 75-. 0 12 24 36 48 60 72 84 96 Time After Injection (h) Figure 3. Change in the hemolymph level of radiolabeled highly polar ecdysteroid metabolites (HP) as a function of time and molt stage. ['H]-ecdysone was injected into lobsters at time zero. Concentration of labeled HP in the hemolymph is expressed as a percentage of the total ['H]-ecdysteroids. Separation and quantification conditions are as listed in Figure I and Materials and Methods. Sample
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 478 M. J. SNYDER AND E. S. CHANG T3 O LJ -C a ^v o E 0) o •*-* c tu Q. 100n 75-. 0 12 24 36 48 60 72 84 96 Time After Injection (h) Figure 3. Change in the hemolymph level of radiolabeled highly polar ecdysteroid metabolites (HP) as a function of time and molt stage. ['H]-ecdysone was injected into lobsters at time zero. Concentration of labeled HP in the hemolymph is expressed as a percentage of the total ['H]-ecdysteroids. Separation and quantification conditions are as listed in Figure I and Materials and Methods. Sample sizes are as given for Table I with the addition of n = 3 for stage A. Bars indicate one standard deviation from the mean. than those of other stages through 96 h. Premolt lobsters in stages D, and D2 had similar 20E percentages at least until 12 h post-injection of [3H]-ecdysone. By 24 h, the [3H]-20E in the D2 lobsters started to drop dramatically, reaching, by 72-96 h, levels equivalent to those in stages A and C. Levels of [3H]-20E were significantly lower in D: than either D0 or D, lobsters by 24 h. The concentra- tions of [3H]-20E in stages A and C changed together throughout the 96 h experiment. Levels for those two stages peaked (41-47%) at 4 h, and then dropped to of the total [3H]-ecdysteroids by 72 h. Stage A and C [3H]-20E levels were significantly lower than those of all other stages until 96 h post-injection, when stage D: lobsters had similar values. These data indicate that the rate of 20E loss becomes significantly faster as lobsters approach stage D,. Changes with time in the percentages of highly polar ecdysteroid metabolites (HP) were the converse of those in 20E (Fig. 3). All molt stages were similar from 1-4 h post-injection of [3H]-ecdysone. The hemolymph per- centages of [3H]-HP in stages D0 and D, lobsters barely increased. This is because [3H]-20E percentages remain high in these two stages through 96 h (Fig. 2). The per- centage of [3H]-H
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
