. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. 248 ELECTRON TRANSPORT response to NAD and NADH concentrations observed by Horio and Kamen in the caseof NADH-RHP reductase and the NAD photoreduction supported by succinate with R. rubnim chromatophores (36,37). The pH optima of these reactions are shown in Fig. 10. The effect of temperature on the photooxidation of TMPD was examined by fol- lowing the reaction at room temperature and at the temperature of liquid nitrogen. These experiments, shown in Fig. 11, were carried out in collaboration with and through the courtesy of
. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. 248 ELECTRON TRANSPORT response to NAD and NADH concentrations observed by Horio and Kamen in the caseof NADH-RHP reductase and the NAD photoreduction supported by succinate with R. rubnim chromatophores (36,37). The pH optima of these reactions are shown in Fig. 10. The effect of temperature on the photooxidation of TMPD was examined by fol- lowing the reaction at room temperature and at the temperature of liquid nitrogen. These experiments, shown in Fig. 11, were carried out in collaboration with and through the courtesy of Dr. Britton Chance, The photooxidation of TMPD did not proceed at the temperature of liquid nitrogen, indicating an ordinary chemical reaction was involved in this photooxidation. This distinguishes it from the physical process which results in cytochrome oxidation in Cliromatium (12).. 440 480 520 560 600 WAVELENGTH, m/x 640 680 Fig. 11. Lack of TMPD photooxidation at 77°K. Experi- mental conditions given for Fig. 3 were used, except that the reactions were run aerobically and contained mg of bacterial chlorophyll. These experiments were performed by Dr. Britton Chance using the spectro- photometer described previously (12). The curve cor- responding to 300°K was taken directly from the re- cording following 30 seconds illumination time with red light. The curve corresponding to 77°K was plotted by taking the difference between the tracing obtained before illumination and following 70-second illumination. The minimum observed at 600 ni^ is characteristic of light absorption changes which occur with chromato- phores Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Symposium on Bacterial Photosynthesis (1963 : Yellow Springs, Ohio); Charles F. Kettering Research Laboratory (Yellow Springs, Ohio
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