. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Guilliermond - Atkinson 64 Cytoplasm especially at the borders of the vacuoles and about the nuclei, where it forms as small islands which soon run together in large masses. This elaboration of glycogen takes place without any direct partici- pation of the chondriome, a fact confirmed by DuCHAUSSOY and Sa- RAZIN. The significance of these vesicles, therefore, is completely unknown. It will be seen that similar vesicles form where there is the slightest alteration in the cell. It might be asked, therefore, whether they are


. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Guilliermond - Atkinson 64 Cytoplasm especially at the borders of the vacuoles and about the nuclei, where it forms as small islands which soon run together in large masses. This elaboration of glycogen takes place without any direct partici- pation of the chondriome, a fact confirmed by DuCHAUSSOY and Sa- RAZIN. The significance of these vesicles, therefore, is completely unknown. It will be seen that similar vesicles form where there is the slightest alteration in the cell. It might be asked, therefore, whether they are not attributable to fixatives. Yet these vesicles always appear at the same stage of development, whatever the fixa- tive employed, and subsequently disappear. It is therefore difficult to accept this opinion, unless it be supposed that the chondriome offers a much greater fragility in that stage of development of the fungi at which they appear. Besides, we shall see that in living Saprolegniaceae similar vesicles may appear and disappear during the course of observation. There is the possibility that these ves- icles indicate an elaboration of a product within the chondriosome which reagents do not reveal. The question remains unanswered for the time being. 0 C.'i'^i oo Fig. Gl / o oo € 6 0. o 'cP® ^%%i Gl It has been demonstrated, moreover, by our work that red pigments (carotinoids), found in the paraphyses of some Ascomycetes and in cells of some yeasts (Sporobolomy- ces), are not formed in the chondriosomes, but are al- ways scattered in small lipide granules having no genet- ic relationship with the chon- driosomes^. The pigments of the Myxomycetes are not con- nected with the chondriosomes either. They are phenol compounds which exist in the cytoplasm as sphaerocrystals (Mangenot). One sees, therefore, that research on fungi, both by observation of living material and with mitochondrial methods, does not confirm the hypothesis formulated for animal cells, namely,


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