. The Biological bulletin. Biology; Zoology; Marine biology. 274 W. I. BROWN AND J. M. SHICK begun hr later. A manometer was attached to each chamber so that pumping activity of the animal was reflected by fluid movements which were detected by a float mechanism and recorded on a kymograph. Oxygen leakage into the apparatus was prevented by sealing the manometer columns with a layer of mineral oil. Po2 determinations were made using a Radiometer-Copenhagen model BMS 3 Mk 2 Blood Micro System attached to a PHM 71 Mk 2 acid-base analyzer. Periodically. ml samples were taken through the m
. The Biological bulletin. Biology; Zoology; Marine biology. 274 W. I. BROWN AND J. M. SHICK begun hr later. A manometer was attached to each chamber so that pumping activity of the animal was reflected by fluid movements which were detected by a float mechanism and recorded on a kymograph. Oxygen leakage into the apparatus was prevented by sealing the manometer columns with a layer of mineral oil. Po2 determinations were made using a Radiometer-Copenhagen model BMS 3 Mk 2 Blood Micro System attached to a PHM 71 Mk 2 acid-base analyzer. Periodically. ml samples were taken through the manometer columns using a long needle and glass syringe; afterwards this same volume was returned to the chamber by injecting air-saturated sea water. A large apparatus of similar design with chamber volumes of 1500 and 530 ml was built to accommodate the larger specimens of Cucumaria frondosa, but was modified by having portals for oxygen electrodes to record oxygen depletion continuously. Sea water was circulated within the chambers by submersible magnetic stirrers for the smaller chambers, or by the self-stirring 02 electrode for the larger unit. Determinations of oxygen uptake via the respiratory trees and general body surface, frequency of pumping, average volume of water moved per pumping cycle, and volume of water moved per hour were made for discrete intervals of P02 over a wide Po2 range. Oxygen extraction efficiency was determined for both species by a direct method, which involved sampling both inhalent and exhalent respirators- currents 200 :100 CD CM 40 O C\J D 20 10. 0-4 1-0 20 Dry weight (grams) 40 Figure 1. Weight-specific rates of oxygen uptake (Vo:) in Sclerodactyla briareus at 10° C as a function of body weight. Closed circles and solid line represent intact animals. Open circles and broken line represent animals with the cloacal opening occluded. Equations relating Vo2 to dry weight (W) for intact animals and cloaca-occluded animals, respectively, are: l
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Keywords: ., bookcentury1800, bookdecade1890, booksubjectb, booksubjectzoology
