. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 106 M. B. SAFFO AND W. L. DAVIS Nephromyces-//-ee Molgula (experiments 1-5) In the laboratory, field-collected adults were rinsed of debris and ambient water, then induced to spawn into ^m-filtered seawater (Saffo, 1978). Embryos were maintained in ^m-filtered seawater through metamorphosis and initiation of feeding by the settled zooids (Fig. 1). California-raised M. manhattensis were grown beyond this stage of development in coarsely-filtered (filtrate with particle size ^ 1 mm) seawater collected from Bodega Bay
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 106 M. B. SAFFO AND W. L. DAVIS Nephromyces-//-ee Molgula (experiments 1-5) In the laboratory, field-collected adults were rinsed of debris and ambient water, then induced to spawn into ^m-filtered seawater (Saffo, 1978). Embryos were maintained in ^m-filtered seawater through metamorphosis and initiation of feeding by the settled zooids (Fig. 1). California-raised M. manhattensis were grown beyond this stage of development in coarsely-filtered (filtrate with particle size ^ 1 mm) seawater collected from Bodega Bay, California, where Molgula (and Nephromyces} are not present. Massachusetts-raised M. manhattensis were grown in ^m-filtered seawater. These laboratory-grown M. manhattensis were fed a variety of axenically cultured unicellular algae, including Dunaliella, Pla- tymonas, Isochrysis, Monochrysis, Stichococcus, and Nannochlorus spp. Inoculation experiments For experiments 1-4, laboratory-raised, Nephromyces-free M. manhattensis zooids were inoculated with Nephromyces by introduction of renal sac contents (including renal sac fluid, and some concretions, as well as Nephromyces cells) freshly dissected from sexually mature, field-collected M. manhattensis, into the ambient water of the settled zooids. After varying periods of exposure to Nephro- myces cells (1 hour to 9 days at 16-19°C), culture dishes on which zooids had settled were rinsed thoroughly with /urn-filtered seawater. In experiment 3, other modes of inoculation were tested as well. Some labo- ratory-raised, Nephromyces-fTQe zooids were incubated at 17°C with living, Ne- phromyces-inhcied M. manhattensis (7-9 days in a one gallon aquarium with one or two field-collected adults; 54 days in a five gallon aquarium with 20 laboratory-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may no
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
