. Carnegie Institution of Washington publication. ABSORBING POWER AND FLUORESCENCE OF RESORUFIN. 27 ;f(r». 3. The effect of diluting a fluorescent solution is similar to that of diminishing the distance through which the fluorescent light travels. METHOD OF OBSERVATION. The instrument used for this work was the spectrophotometer of Lummer and Brodhun. The source of light was an acetylene flame 6" (Fig. 22) from which light was diffusely reflected by a block of magnesium carbonate, n, mounted at an angle at 45° with axis of the collimator A. For observations on transmission a s
. Carnegie Institution of Washington publication. ABSORBING POWER AND FLUORESCENCE OF RESORUFIN. 27 ;f(r». 3. The effect of diluting a fluorescent solution is similar to that of diminishing the distance through which the fluorescent light travels. METHOD OF OBSERVATION. The instrument used for this work was the spectrophotometer of Lummer and Brodhun. The source of light was an acetylene flame 6" (Fig. 22) from which light was diffusely reflected by a block of magnesium carbonate, n, mounted at an angle at 45° with axis of the collimator A. For observations on transmission a similar block of magnesium carbonate was placed in front of the slit D. The work was performed in a room with black walls, the acetylene flame being the only source of light. Screens were adjusted so that no stray light could strike any part of the instrument. The solution under consideration was placed in front of slit D, the width of which was kept constant during a single set of observations. The ocular lenses of the telescope were removed, the eye being focused upon the face of the prism magnified by the objec- tive of the telescope T. In making observations the width of slit C was adjusted by means of a micrometer screw until the central band of light reflected from KL was brought into the best possible coinci- 1âytlT dence with that transmitted from D through the upper and lower parts of the cube. The results given represent an average of from 5 to 10 settings, the zero point of the screw attached to C being carefully determined and care being taken to avoid errors due to lost motion. The hydrogen lines of a vacuum Fig tube, together with a few of the Fraun- hofer lines, were used for 22.âApparatus arranged for measuring fluorescence. COMPOSITION OF RESORUFIN. Weselski's diazo-resorufin,1 the fluorescent substance used in this investi- gation,2 has the formula I ' c o c â: 1 p . I '_ I â Berichte der deutschen chetnischen Gesellschaft, 22, 3036. 2 Ack
Size: 1877px × 1332px
Photo credit: © Book Worm / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorcarnegie, bookcentury1900, bookdecade1910, bookyear1912
