. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 260 S. K. GOFFREDI ET AL CO2 min"1 mg protein ' (n = 27) and ± jumol CO2 min"1 mg protein"' (n = 16), respectively. Internal vs. external parameter experiments All experimental worms were first kept in maintenance vessels with surface seawater conditions, and monitored until extracellular fluid SCO2 was reduced to a low value (<10 mmol r1; about 2-4 days). When control worms were subsequently exposed to flowing water with an aver- age 2CO2 of ± mmol 1~' and Pco, of ± kPa (typical v
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 260 S. K. GOFFREDI ET AL CO2 min"1 mg protein ' (n = 27) and ± jumol CO2 min"1 mg protein"' (n = 16), respectively. Internal vs. external parameter experiments All experimental worms were first kept in maintenance vessels with surface seawater conditions, and monitored until extracellular fluid SCO2 was reduced to a low value (<10 mmol r1; about 2-4 days). When control worms were subsequently exposed to flowing water with an aver- age 2CO2 of ± mmol 1~' and Pco, of ± kPa (typical vent conditions), they demonstrated an increase in extracellular fluid SCO2 (Fig. la. b). The rate of increase in internal SCO2, from 0 to 6 h, was mmol P'h"1 for coelomic fluid and mmol l~'h~' for vascular blood. Despite this increase in internal SCO2, there was no change in the pH of either coelomic fluid or vascular blood; values averaged ± and ± , respectively, during the 6-h time period (Fig. Ic, d). Worms exposed to EZ were kept in the same overall water conditions as the control worms, with an additional 1 h exposure to mmol 1~' EZ. These worms, when placed in typical vent conditions ( high SCO2 and PCO,)- did not accumulate internal SCO2 as rapidly as the control worms ( mmol and mmol 1 for coelomic fluid and vascular blood, respectively: Fig. la, b). The decreased rate of iCCK accumulation for vascular blood in the EZ-exposed worms was significantly less than that of the control worms (P = , ANCOVA; Fig. Ib). EZ-exposed worms, like the control worms, showed no change in the pH of either coelomic fluid or vascular blood during the experiment ( ± and ± , respectively), and neither was significantly different from that of the control worms (P = and P = , respec- tively, Mann-Whitney U test; Fig. Ic, d). EZ exposure also resulted in a decrease in CA activity of about 99%: from
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
