. A text-book of mycology and plant pathology . Plant diseases; Fungi in agriculture; Plant diseases; Fungi. 624 LABORATORY EXERCISES LESSON 22 Isolation oj a Leaf Will Fungus in Pure Culture—Givea a fungus causing leaf wilt, to obtain a pure culture by excluding the non-pathogenic forms. I. Look for the fruiting stage of the suspected fungus, or fungi. Transfer some of the spores with a sterile needle into a tube of s of sterile water. (If pycnidia or perithecia are present, transfer a whole pycnidium or perithecium into sterile water, and crush the fruit body to cause the escape of the
. A text-book of mycology and plant pathology . Plant diseases; Fungi in agriculture; Plant diseases; Fungi. 624 LABORATORY EXERCISES LESSON 22 Isolation oj a Leaf Will Fungus in Pure Culture—Givea a fungus causing leaf wilt, to obtain a pure culture by excluding the non-pathogenic forms. I. Look for the fruiting stage of the suspected fungus, or fungi. Transfer some of the spores with a sterile needle into a tube of s of sterile water. (If pycnidia or perithecia are present, transfer a whole pycnidium or perithecium into sterile water, and crush the fruit body to cause the escape of the spores). Then with a sterile needle transfer some of the water with the spores into a tube of agar-agar which is made liquid by putting in a vessel of hot water and then allowed to cool. This tube is marked A. Then from tube .4 transfer a drop of agar with a sterile needle to another similar test-tube with liquid agar designated as B (Fig. 221). Then perform the same sort of transfer to a third tube C. Distilled water or nutrient bouillon can be used for these dilutions instead of Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Harshberger, John W. (John William), 1869-1929. Philadelphia : P. Blakiston's Son & Co
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectfungi, bookyear1917
