. Sacbrood. Bees. 42 BULLETIN 431, V. S. DBPABTMENT OF AGBICULTUBE. A small quantity of soil is added to inoculate the suspension further. This is then distributed in test tubes (fig. 33), the quantity in each tube representing the virus from about 15 larvae. These suspensions are allowed to remain at room temperature, shielded from the light. Under these conditions fermenta- tion goes on rather rapidly. After intervals reckoned in days colonies free from the disease are inoculated, each with the suspension from a single tube. Results from such inoculations are given in the following table:. ^
. Sacbrood. Bees. 42 BULLETIN 431, V. S. DBPABTMENT OF AGBICULTUBE. A small quantity of soil is added to inoculate the suspension further. This is then distributed in test tubes (fig. 33), the quantity in each tube representing the virus from about 15 larvae. These suspensions are allowed to remain at room temperature, shielded from the light. Under these conditions fermenta- tion goes on rather rapidly. After intervals reckoned in days colonies free from the disease are inoculated, each with the suspension from a single tube. Results from such inoculations are given in the following table:. ^"i;^. Table IX.—Resistance of sachrood virus to fermentation in a 10 per cent sugar solution at room temperature. Date ot inoculation. Sept. 9, Sept. 11, Do Sept. 13, July 14, July 22, Sept. 14, Sept. 22,1915. July 10, June 10, July 7, Aug. 27, Do Do Do Do Period ot fermen- tatioa. Days. 1 2- 3 i 3 5 5 7 9 13 34 61 85 87 90 244 Results of inoculation. Saobrood produced. Bo. Do. Do. No disease produced. Do. Do. Do. Do. Do. Do. Do. Do. Do. Do. Do. Fig. 33.—Test tube bearing a cotton plug, used in testing tbe ef- fect of fermentation, putrefaction, and dis- infecting agents on the virus of sacbrood. (Original.) • Theresultsrecorded for 1914 were obtained with a suspension of crusbed larvae, in various stages ot decay, in sirup made from about equal parts water and sugar. From the results of experimenfs recorded in Table IX it wiU be noted that the virus of sacbrood was destroyed in from three to five days in the presence of fermentation in 10 per cent cane sugar (saccharose) at room temperature. As the rapidity of fermentative processes varies with the temperature present, it is natural to sup- pose that the time required for the destruction of the virus wiU vary. From experiments it is found that at incubator temperature the time is slightly less, and at outdoor temperature it is somewhat greater than at
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