. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ASCIDIAN HEMAGGLUTININ 145 weight molecular substance. The rough molecular weight was estimated by the gel-filtration on Sephadex. The coelomic fluid wras heated at 100° C for 15 minutes, then centrifuged to remove the precipitate and applied to Sephadex G-100 column. A typical elution pattern was shown in Figure 1. The same experiment was done using the Sephadex G-200 column. As shown in Figure 1, two peaks appeared, and hemagglutinic activity was found at both peaks when Sephadex G-200 was used. The coelomic fluid seems to
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ASCIDIAN HEMAGGLUTININ 145 weight molecular substance. The rough molecular weight was estimated by the gel-filtration on Sephadex. The coelomic fluid wras heated at 100° C for 15 minutes, then centrifuged to remove the precipitate and applied to Sephadex G-100 column. A typical elution pattern was shown in Figure 1. The same experiment was done using the Sephadex G-200 column. As shown in Figure 1, two peaks appeared, and hemagglutinic activity was found at both peaks when Sephadex G-200 was used. The coelomic fluid seems to contain two molecular species of hemagglutinin. Assuming that the hemagglutinin is polysaccharide, the molecular weight of the smaller one could lie between 150,000 and and the larger one be over 800,000. 0-5 E o GO C\l Q O Sephadex-GlOO. Sephadex-G200. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
