. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 7. Examples of srDNA heterogeneity within samples of Sym- biodinium E. srDNAs were amplified (with host-excluding PCR primers) from different samples of Symbiodinium E (lanes 1-3) and from srDNA clones E0'1, E0'2, and E0"-1 (as indicated) and then digested with Dpn II. Mae III. Mnl I. and Alu I (indicated on the left). On the right, arrows identify the positions of additional DNA fragments in lanes 1-3 that indicate srDNA heterogeneity (see text). In Mae III and Mnl I digestions, these bands were also observed in
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 7. Examples of srDNA heterogeneity within samples of Sym- biodinium E. srDNAs were amplified (with host-excluding PCR primers) from different samples of Symbiodinium E (lanes 1-3) and from srDNA clones E0'1, E0'2, and E0"-1 (as indicated) and then digested with Dpn II. Mae III. Mnl I. and Alu I (indicated on the left). On the right, arrows identify the positions of additional DNA fragments in lanes 1-3 that indicate srDNA heterogeneity (see text). In Mae III and Mnl I digestions, these bands were also observed in one of the three clones; for A/u I digestions no clone contains the indicated band. Samples are from Mon- tastraea franksi (lane 1), from (lane 2), and fromM. annuluris (lane 3). Lane M contains DNA size markers as in Figure 2. samples were also heterogeneous. However, unlike Symbio- dinium E and B (above), within-sample srDNA heterogene- ity in Symbiodinium C varied both qualitatively (, com- pare samples 3-5 in Dpn II panel. Fig. 9) and quantitatively (, compare Samples 1-5 in Hinfl panel, Fig. 9) among samples. srDNA heterogeneity was observed in as few as one or as many as six different digests (examples in Fig. 9) among the 12 samples tested. That additional variation suggested that some or all samples might have contained more than one genotype of Symbiodinium. We made two analyses that might have supported this hypothesis. First, because mixtures of Symbiodinium A, B, C, or E vary in proportion at different locations within a coral colony (Rowan and Knowlton. 1995; Rowan et 1997; Results), we analyzed multiple samples from colonies of M. iinnuliiris in which Symbiodinium C had been ob- served previously. In 14 colonies (each consisting of a cluster of columns), we sampled one column on its top and on its side; srDNA genotypes were indistinguishable in every top-versHs-side comparison (not shown). We also sampled the tops of one or two additional
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