Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . wo instruments of different make will agree, but com-parative results sufficiently accurate for chnical purposes are obtained by theuse of the more reliable instruments. The personal equation in reading the THE BLOOD. 417 color comparisons must be remembered, as some individuals show abnormalsensitiveness or lack of sensitiveness to shadings of red. It is an impossibilityin this book to give in detail all of the methods advanced for the estimation ofhemoglobin. I select, therefore, tho
Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . wo instruments of different make will agree, but com-parative results sufficiently accurate for chnical purposes are obtained by theuse of the more reliable instruments. The personal equation in reading the THE BLOOD. 417 color comparisons must be remembered, as some individuals show abnormalsensitiveness or lack of sensitiveness to shadings of red. It is an impossibilityin this book to give in detail all of the methods advanced for the estimation ofhemoglobin. I select, therefore, those that have proven most reliable in my hands. Hemometer of Fleischl-Miescher. Up to recent times the most frequently used of the instruments for theestimation of hemoglobin was the old von Fleischl instrument. With theintroduction of the Miescher modification, this original form has been or shouldbe less often employed. We avoid, therefore, a discussion of the older instru-ment, referring to other works which have included it. This new apparatus, made by Reichert, under the direction of Miescher, D >. Fig. 125.—Hemometer of Fleischl-Miescher: R, Stage; T, milled head, which movesthe color scale; m, opening in stage through which the instrument is read; M, mixing cell;D, cover glass; D, cap; PS, gypsum mirror from which light is reflected; mel, dilutingpipet. is similar in general appearance to the old von Fleischl. It has the same standand the same scale principle, although this latter is standardized differently andgraduated on a different basis. It differs, materially, in the method of measur-ing and diluting the blood, in the form of the comparison chamber, and in themeaning of the graduation of the scale (see cut and legend for its description). The Diluting Pipet. This is similar in construction to the pipet of the Thoma-Zeiss hemo-cytometer, its calibrations, however, being different. The marks are 1/2,2/3, and I. Above and below each of these main divisions are two
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