. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 266 J. W. HASTINGS AND GEORGE MITCHELL I I 1 1 1 1 1. 440 460 480 500 WAVE LENGTH- 520 540 FIGURE 3. Bioluminescent emission spectra for the in riro emission from a symbiont (L-4; A) and an isolate from Sek Harbor ( SH-1 ; o). Using dodecanal, the first order constant (k) for the decay of luminescence was far more rapid for one (strain Pf: Plwtobactcriiun fischeri, ATCC 7744; k = sec'1) than for the other (strain MAV; k — sec'1)- On the other hand, using decanal the rate constants were similar for the two luciferase
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 266 J. W. HASTINGS AND GEORGE MITCHELL I I 1 1 1 1 1. 440 460 480 500 WAVE LENGTH- 520 540 FIGURE 3. Bioluminescent emission spectra for the in riro emission from a symbiont (L-4; A) and an isolate from Sek Harbor ( SH-1 ; o). Using dodecanal, the first order constant (k) for the decay of luminescence was far more rapid for one (strain Pf: Plwtobactcriiun fischeri, ATCC 7744; k = sec'1) than for the other (strain MAV; k — sec'1)- On the other hand, using decanal the rate constants were similar for the two luciferases. Luciferases isolated from the symbiotic pony fish bacterium (L-4) and from the "free living" vSek Harbor strain (SH-1) were found to correspond in their kinetics (Fig. 2) to either one or the other of the original two types of Hastings, Weber, Friedland, Eberhard, Mitchell and Gunsalus (1969), not only with dodecanal, but with decanal as well. In order to determine whether or not any specificity with regard to these luciferases is involved among the bacteria which occur as symbionts, isolates from twenty additional pony fish were obtained, purified, grown and extracted. The luciferase of all was found to be of the fast (Pf) type. Of the twenty-one additional "free living" isolates from Sek Harbor, fifteen possessed "slow" (MAV) luciferase, similar to SH-1. The remaining six appeared to be similar to the symbionts with regard to their luciferase. The color of the in vivo bioluminescence was also found to be slightly different for the two isolates (Fig. 3). The emission from two of the Sek Harbor strains peaked at about 485 nm, slightly to the blue as compared to the light from two symbionts measured (A max 491 nm). One of the harbor isolates which had sym- biont-like luciferase was tested and found to be similar to the symbionts in the color of in z*ivo emission. DISCUSSION From previous evidence, one might always have argued that the bacteria-
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
