. Annual report. Entomological Society of Ontario; Insect pests; Insects. 74 THE REPORT OF THE. Plate 2.—Rearing Jars. Type of jar used for maturing the flies. Separate items (left to right), (1) Jar of pupating larvae, (2) Empty jar, (3) Jar freshly charged showing wheat bran in front end, and culture medium plus eggs and larvae at back end. The cages of aging flies are kept in a large constant-temperature chamber thermostatically controlled at 70 deg. F. Lantern Globe Spray Chamber.—The flies are sprayed in closed lantern globes. As many as 1000 Drosophila melanogaster may be put in a globe
. Annual report. Entomological Society of Ontario; Insect pests; Insects. 74 THE REPORT OF THE. Plate 2.—Rearing Jars. Type of jar used for maturing the flies. Separate items (left to right), (1) Jar of pupating larvae, (2) Empty jar, (3) Jar freshly charged showing wheat bran in front end, and culture medium plus eggs and larvae at back end. The cages of aging flies are kept in a large constant-temperature chamber thermostatically controlled at 70 deg. F. Lantern Globe Spray Chamber.—The flies are sprayed in closed lantern globes. As many as 1000 Drosophila melanogaster may be put in a globe and sprayed at one time. However, approximately 300 flies has been found to be a very satisfactory number to handle in a globe. If too many flies are used at a time, it is difficult to get an accurate count of the dead if the mortality rate is high. The globe is closed at the large (lower) end with a good grade of dusting cotton, securely fastened in place by means of a wire ring. The same kind of cloth is used to cover the upper end and is held in place with an elastic band. A y8 inch hole in the cloth in the top allows for the entry of flies and also for the insertion of an inverted feeding vial filled with a 3 per cent honey solution and stoppered with cotton batting. The feeding vial is left in position except during the entry of flies and when spraying. Flies may be aged in the globes rather than in an aging cage. In some of our work it has been found more convenient to do so; particularly when we want to keep separate records on flies taken from different jars. Spray Apparatus.—This consists of an atomizer, arranged to spray vertically upwards, an air pump run by an electric motor, an air tank equipped with a device for keeping the air pressure constant, and a pres- sure gauge so that the operator may know at all times whether the re- quired pressure is being maintained. The atomizer is the Tattersfield type No. 2 as described in the Annals of Applied Biology, Volum
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Keywords: ., bookcentury1800, bookdecade1870, booksubjectinsects, bookyear1872
