. Aquatic surveys and re-assessment of sites within the Middle Powder River Watershed. Aquatic ecology; Aquatic biology; Invertebrates; Mayflies; Coalbed methane. Moorhead gauging station for these calculations. The summation of individual fish species and tolerance metrics range between 0 and 100. Bramblett et al. (2005) did not propose threshold criteria for good, fair, and poor biological integrity for these scores, but instead relied on comparisons to "reference condition" scores. Therefore, we followed Confluence Consulting (2004) methods by applying commonly used criteria of 75


. Aquatic surveys and re-assessment of sites within the Middle Powder River Watershed. Aquatic ecology; Aquatic biology; Invertebrates; Mayflies; Coalbed methane. Moorhead gauging station for these calculations. The summation of individual fish species and tolerance metrics range between 0 and 100. Bramblett et al. (2005) did not propose threshold criteria for good, fair, and poor biological integrity for these scores, but instead relied on comparisons to "reference condition" scores. Therefore, we followed Confluence Consulting (2004) methods by applying commonly used criteria of 75 to 100 indicating good to excellent biological integrity, 25 to 74 indicated fair biological integrity, and less than 25 indicating poor biological integrity in describing condition. Derivation of the expected fish communities is performed by identifying the frequency of occurrence that a species has at a site classified in a reference condition and summing the frequencies across all fish species of the community (see Stagliano 2006). The O/E (Observed taxa of an evaluated site/Expected Taxa for a reference site) model is a direct measure of the community completeness. Taxonomic completeness is a fundamental aspect of biological integrity and is defined here as the proportion of the taxa that "should" occur in a sample (E) that were actually sampled (O) (Jessup et al. 2005). It compares the fish species that are expected at a site with the actual taxa that were found when the site was sampled (carp/introduced species are never "expected" and thus were given scores of zero). Values of the O/E range from 0 to 1, with values of 1 implying reference conditions and values less than 1 implying some form of biological impairment. In some cases, it is more ecologically meaningful than the IBI, but not always. Pairs of fish community samples were compared across years for significant differences by using proportional and taxa community similarity indices (Brower and


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Keywords: ., bhlcoll, bookleafnumber15, booksubjectinvertebrates, bookyear2012