. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. UV-ABSORBING CELLS IN DIDEMNID ASCIDIANS HOSTING UT ZC BT. UV-UT UV-BT Figure 2. Visible- and ultraviolet-light micrographs of a cross-section of a living colonial ascidian. sp. The ascidian sample was collected at Akajima, an island in the Ryukyu Archipelago, Japan. Thin slices of living specimens were cut with a razorblade by hand. They were placed on a quartz slide and immersed in tillered seawater surrounded by glycerol, covered with a quartz coverslip. and observed under a light microscope with N
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. UV-ABSORBING CELLS IN DIDEMNID ASCIDIANS HOSTING UT ZC BT. UV-UT UV-BT Figure 2. Visible- and ultraviolet-light micrographs of a cross-section of a living colonial ascidian. sp. The ascidian sample was collected at Akajima, an island in the Ryukyu Archipelago, Japan. Thin slices of living specimens were cut with a razorblade by hand. They were placed on a quartz slide and immersed in tillered seawater surrounded by glycerol, covered with a quartz coverslip. and observed under a light microscope with Nikon Fluor objectives (10X NA = , 20x NA = ). The glass eyepiece and condenser lens were removed, because they absorb UV light. An interference filter (bandpass) for 319 ± II nm (median ± 50% maximum transparency) or for 331 ± 5 nm (median ± 50% maximum transparency) was inserted between a reflex camera body (Olympus OM-2) mounted on the microscope and the objective lens. The light source was a UV lamp (Vilber-Lourmat T15-N, France: or Toshiba F6T5 UV-B lamp, Japan). Black-and-white film (Fuji Neopan F) was used to record the images. Because the focal plane for the UV light is different from that for visible light, it was determined before the experiment by making test exposures. For visible-light microscopy, the interference UV bandpass filter was removed from the light path, and an UV-opaque filter was inserted between the light source and the specimen. (A) Visible-light micrograph; (B) UV-light micrograph (320 nm). UT, upper tunic layer: BT. basal tunic layer; ZC, zooid and cloacal cavity layer; UV-UT, UV-light-absorbing zone in the upper tunic; UV-BT, UV-light-absorbing zone in the basal tunic. Scale. 200 fum. and analyzed the extracts for MAAs by reversed-phase liquid chromatography according to Dionosio-Sese ci al. (10); the MAAs from another ascidian, Halocyntliiu mretzi, were used as references (20). The major MAA (about 94%) was shinorine. and the minor MAA
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